論文 - 詳細
| RRC ID | 6287 |
|---|---|
| 著者 | Simkhada D, Kim E, Lee HC, Sohng JK. |
| タイトル | Metabolic engineering of Escherichia coli for the biological synthesis of 7-O-xylosyl naringenin. |
| ジャーナル | Mol Cells |
| Abstract |
Flavonoids are a group of polyphenolic compounds that have been recognized as important due to their physiological and pharmacological roles and their health benefits. Glycosylation of flavonoids has a wide range of effects on flavonoid solubility, stability, and bioavailability. We previously generated the E. coli BL21 (DE3) Deltapgi host by deleting the glucose-phosphate isomerase (Pgi) gene in E. coli BL21 (DE3). This host was further engineered for whole-cell biotransformation by integration of galU from E. coli K12, and expression of calS8 (UDP-glucose dehydrogenase) and calS9 (UDP-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-4 (7-O-glycosyltransferase) from Arabidopsis thaliana to form E. coli (US89Gt-4), which is expected to produce glycosylated flavonoids. To test the designed system, the engineered host was fed with naringenin as a substrate, and naringenin 7-O-xyloside, a glycosylated naringenin product, was detected. Product was verified by HPLCLC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids. |
| 巻・号 | 28(4) |
| ページ | 397-401 |
| 公開日 | 2009-10-31 |
| DOI | 10.1007/s10059-009-0135-7 |
| PMID | 19812897 |
| MeSH | Anti-Ulcer Agents Antineoplastic Agents Escherichia coli / genetics Escherichia coli / metabolism* Estrogen Antagonists Flavanones / biosynthesis* Genetic Engineering / methods* Glycosylation Mass Spectrometry Metabolomics Organisms, Genetically Modified Xylose / analogs & derivatives* Xylose / biosynthesis |
| IF | 4.081 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda09852 |