RRC ID 6302
Author Noro Y, Takano-Shimizu T, Syono K, Kishima Y, Sano Y.
Title Genetic variations in rice in vitro cultures at the EPSPs-RPS20 region.
Journal Theor. Appl. Genet.
Abstract In vitro cultures of plant cells have often been utilized to generate genetic variations, which are designated somaclonal variations. Little is known about the major genetic alterations in the cultured cells and the nature of these genetic changes. Here, we examined different lines of rice Oc cells that have been cultured for more than 20 years on agar media or in liquid media. We surveyed 35 clones obtained from PCR amplification of the 3-kb EPSPs-RPS20 region. The sequence divergence among the Oc cells was even greater than that between Japonica and Indica rice cultivars. The divergent sequences appeared to be maintained as multiple copies in a single cell. Surprisingly, the nucleotide substitutions in the Oc cells were characterized by an extremely high frequency of transition mutations of A/T-to-G/C, a feature which is similar to that of the mutations caused by chemical mutagens such as 5-bromouracil and 2-aminopurine. Although no replacements in the exons of this region were observed among the AA-genome Oryza species, our results revealed that the nucleotide substitutions of the cultured cell lines occurred more frequently at replacement sites in the exons than at synonymous sites. These distinct mutation biases found in rice in vitro cultures might contribute importantly to somaclonal variations.
Volume 114(4)
Pages 705-11
Published 2007-2
DOI 10.1007/s00122-006-0470-4
PMID 17177062
MeSH Base Sequence DNA Primers Genes, Plant / genetics* Genetic Variation* Molecular Sequence Data Mutation / genetics* Oryza / genetics* Sequence Analysis, DNA Species Specificity
IF 3.926
Times Cited 17
WOS Category HORTICULTURE AGRONOMY PLANT SCIENCES GENETICS & HEREDITY
Resource
Arabidopsis / Cultured plant cells, genes rpc00031