論文 - 詳細
| RRC ID | 63024 |
|---|---|
| 著者 | Iio A, Kaji K, Kaji N, Hori M, Yonezawa T, Momoi Y, Maeda S. |
| タイトル | Expression analysis of protease-activated receptor-2 in cats. |
| ジャーナル | Vet Immunol Immunopathol |
| Abstract |
Chronic kidney disease (CKD) is a common disease in geriatric cats. Despite its high prevalence, the pathogenesis of feline CKD is poorly understood. Recently, there has been increasing evidence for the role of protease-activated receptor-2 (PAR-2) in the progression of CKD in humans and rodents. However, the role of PAR-2 in feline CKD has not been evaluated. In this study, we determined nucleotide sequence of feline PAR-2 from the kidney, evaluated PAR-2 mRNA and protein expression in normal feline tissues, and analyzed functional expression in the feline kidney epithelial cell line Crandell-Rees Feline Kidney (CRFK). The open reading frame of feline PAR-2 comprised 1,194 bp and encoded 397 amino acids, showing 90%, 90%, and 85% identities to human, dog, and mouse PAR-2, respectively. In healthy cats, expression levels of the PAR-2 mRNA and protein were relatively higher in the gastrointestinal tract and kidney, and was lowest in the heart. The feline PAR-2 protein expression was confirmed, and stimulation of trypsin and PAR-2 agonists induced a prompt increase in the intracellular calcium ion concentration in CRFK cells. The present study will provide fundamental information for investigation of the involvement of PAR-2 in the pathogenesis of CKD in cats. |
| 巻・号 | 229 |
| ページ | 110115 |
| 公開日 | 2020-11-1 |
| DOI | 10.1016/j.vetimm.2020.110115 |
| PII | S0165-2427(20)30141-0 |
| PMID | 32932190 |
| MeSH | Animals Cat Diseases / genetics Cat Diseases / metabolism* Cats Cell Line DNA, Complementary HEK293 Cells Humans Receptor, PAR-2 / biosynthesis* Receptor, PAR-2 / genetics Receptor, PAR-2 / metabolism Renal Insufficiency, Chronic / genetics Renal Insufficiency, Chronic / metabolism Renal Insufficiency, Chronic / veterinary* Sequence Analysis, DNA Tissue Distribution Transcriptome Trypsin / metabolism |
| IF | 1.713 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |