論文 - 詳細
| RRC ID | 63044 |
|---|---|
| 著者 | Watanabe T, Kakuta J, Saito S, Hasehira K, Kiyoi K, Imai T, Tateno H. |
| タイトル | Monoclonal antibodies specific for podocalyxin expressed on human induced pluripotent stem cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Human induced pluripotent stem cells (hiPSCs) are useful starting materials for the generation of cell therapy products, due to their pluripotency and ability to self-renew. Quality control of hiPSCs is extremely important in creating a stable supply of hPSC-derived products. Previously we identified an hiPSC-specific lectin probe, rBC2LCN, which binds specifically to α1,2-fucosylated glycan and recognizes podocalyxin (PODXL) as a glycoprotein ligand. In this study, we produced monoclonal antibodies (mAbs) specific for α1,2-fucosylated PODXL expressed on hiPSCs. PODXL was recombinantly expressed in fucosyltransferase 1 (FUT1)-transfected HEK293, followed by immunization into mice. Monoclonal antibodies, which bind to PODXL/FUT1-transfected cells, but not to cells transfected with only one of PODXL or FUT1, were screened by flow cytometry. The two mAbs generated (179-6B8C9 and 179-7E12E10), termed α1,2-fucosylated PODXL-specific mAbs (FpMabs), showed binding specificity to PODXL/FUT1-transfected cells. The FpMabs bound to hiPSCs but never to human adipose-derived mesenchymal stem cells, human dermal fibroblasts, or hiPSC-derived mesoderm. Altogether, FpMabs are highly specific probes for hiPSCs, which might be a powerful tool for the characterization of hiPSCs used in regenerative medicine. |
| 巻・号 | 532(4) |
| ページ | 647-654 |
| 公開日 | 2020-11-19 |
| DOI | 10.1016/j.bbrc.2020.08.092 |
| PII | S0006-291X(20)31692-2 |
| PMID | 32912628 |
| MeSH | Animals Antibodies, Monoclonal* Cell Line Cells, Cultured Flow Cytometry Fluorescence Fucosyltransferases / genetics HEK293 Cells Humans Induced Pluripotent Stem Cells / immunology* Induced Pluripotent Stem Cells / metabolism Mice Polysaccharides / analysis Polysaccharides / immunology Sialoglycoproteins / genetics Sialoglycoproteins / immunology* Sialoglycoproteins / metabolism Transfection |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |