論文 - 詳細
| RRC ID | 63077 |
|---|---|
| 著者 | Shih JH, Tsai YF, Li IH, Chen MH, Huang YS. |
| タイトル | Hp-s1 Ganglioside Suppresses Proinflammatory Responses by Inhibiting MyD88-Dependent NF-κB and JNK/p38 MAPK Pathways in Lipopolysaccharide-Stimulated Microglial Cells. |
| ジャーナル | Mar Drugs |
| Abstract |
Hp-s1 ganglioside is isolated from the sperm of sea urchin (Hemicentrotus pulcherrimus). In addition to neuritogenic activity, the biological function of Hp-s1 in neuroinflammation is unknown. In this study, we investigated the anti-neuroinflammatory effect of Hp-s1 on lipopolysaccharide (LPS)-stimulated microglial cells. MG6 microglial cells were stimulated with LPS in the presence or absence of different Hp-s1 concentrations. The anti-inflammatory effect and underlying mechanism of Hp-s1 in LPS-activated microglia cells were assessed through a Cell Counting kit-8 assay, Western blot analysis, and immunofluorescence. We found that Hp-s1 suppressed not only the expression of inducible nitric oxide synthase and cyclooxygenase-2 but also the expression of proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6. Hp-s1 inhibited the LPS-induced NF-κB signaling pathway by attenuating the phosphorylation and translocation of NF-κB p65 and by disrupting the degradation and phosphorylation of inhibitor κB-α (IκBα). Moreover, Hp-s1 inhibited the LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK). Hp-s1 also reduced the expression of myeloid differentiation factor 88 (MyD88) and TNF receptor-associated factors 6 (TRAF6), which are prerequisites for NF-κB and MAPKs activation. These findings indicated that Hp-s1 alleviated LPS-induced proinflammatory responses in microglial cells by downregulating MyD88-mediated NF-κB and JNK/p38 MAPK signaling pathways, suggesting further evaluation as a new anti-neuroinflammatory drug. |
| 巻・号 | 18(10) |
| 公開日 | 2020-9-29 |
| DOI | 10.3390/md18100496 |
| PII | md18100496 |
| PMID | 33003399 |
| PMC | PMC7600735 |
| MeSH | Animals Anti-Inflammatory Agents / isolation & purification Anti-Inflammatory Agents / pharmacology* Cell Line Cytokines / metabolism Gangliosides / isolation & purification Gangliosides / pharmacology* Hemicentrotus / metabolism Inflammation / drug therapy* Inflammation / pathology Lipopolysaccharides MAP Kinase Signaling System / drug effects Mice Microglia / drug effects* Microglia / pathology Myeloid Differentiation Factor 88 / metabolism NF-KappaB Inhibitor alpha / metabolism NF-kappa B / metabolism Signal Transduction / drug effects p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 4.073 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MG6(RCB2403) |