RRC ID 63215
Author Rico P, Rodrigo-Navarro A, Sánchez Pérez L, Salmeron-Sanchez M.
Title Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway.
Journal Commun Biol
Abstract The intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the use of growth factors. Addition of borax alone, transported by the NaBC1-transporter, enhanced MSC adhesion and contractility, promoted osteogenesis and inhibited adipogenesis. Stimulated-NaBC1 promoted osteogenesis via the BMP canonical pathway (comprising Smad1/YAP nucleus translocation and osteopontin expression) through a mechanism that involves simultaneous NaBC1/BMPR1A and NaBC1/α5β1vβ3 co-localization. We describe an original function for NaBC1 transporter, besides controlling borate homeostasis, capable of stimulating growth factor receptors and fibronectin-binding integrins. Our results open up new biomaterial engineering approaches for biomedical applications by a cost-effective strategy that avoids the use of soluble growth factors.
Volume 3(1)
Pages 717
Published 2020-11-27
DOI 10.1038/s42003-020-01449-4
PII 10.1038/s42003-020-01449-4
PMID 33247189
PMC PMC7695834
MeSH Adipogenesis / drug effects Animals Anion Transport Proteins / metabolism* Bone Morphogenetic Protein Receptors, Type I / metabolism Borates / pharmacology* Cell Nucleus / metabolism Cells, Cultured Integrins / metabolism MAP Kinase Signaling System / drug effects Mice Myosin Light Chains / metabolism Osteogenesis / drug effects* Phosphorylation Smad1 Protein / metabolism Symporters / metabolism*
IF 4.165
Human and Animal Cells 10T1/2(RCB0247)