| RRC ID |
63215
|
| 著者 |
Rico P, Rodrigo-Navarro A, Sánchez Pérez L, Salmeron-Sanchez M.
|
| タイトル |
Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway.
|
| ジャーナル |
Commun Biol
|
| Abstract |
The intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the use of growth factors. Addition of borax alone, transported by the NaBC1-transporter, enhanced MSC adhesion and contractility, promoted osteogenesis and inhibited adipogenesis. Stimulated-NaBC1 promoted osteogenesis via the BMP canonical pathway (comprising Smad1/YAP nucleus translocation and osteopontin expression) through a mechanism that involves simultaneous NaBC1/BMPR1A and NaBC1/α5β1/αvβ3 co-localization. We describe an original function for NaBC1 transporter, besides controlling borate homeostasis, capable of stimulating growth factor receptors and fibronectin-binding integrins. Our results open up new biomaterial engineering approaches for biomedical applications by a cost-effective strategy that avoids the use of soluble growth factors.
|
| 巻・号 |
3(1)
|
| ページ |
717
|
| 公開日 |
2020-11-27
|
| DOI |
10.1038/s42003-020-01449-4
|
| PII |
10.1038/s42003-020-01449-4
|
| PMID |
33247189
|
| PMC |
PMC7695834
|
| MeSH |
Adipogenesis / drug effects
Animals
Anion Transport Proteins / metabolism*
Bone Morphogenetic Protein Receptors, Type I / metabolism
Borates / pharmacology*
Cell Nucleus / metabolism
Cells, Cultured
Integrins / metabolism
MAP Kinase Signaling System / drug effects
Mice
Myosin Light Chains / metabolism
Osteogenesis / drug effects*
Phosphorylation
Smad1 Protein / metabolism
Symporters / metabolism*
|
| IF |
4.165
|
| リソース情報 |
| ヒト・動物細胞 |
10T1/2(RCB0247) |
