RRC ID 63351
Author Yoshikawa-Murakami C, Mizutani Y, Ryu A, Naru E, Teramura T, Homma Y, Fukuda M.
Title A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues.
Journal Int J Mol Sci
Abstract Melanin incorporated into keratinocytes plays an important role in photoprotection; however, abnormal melanin accumulation causes hyperpigmentary disorders. To understand the mechanism behind the accumulation of excess melanin in the skin, it is essential to clarify the spatial distribution of melanosomes or melanin in the epidermis. Although several markers have been used to detect melanosomes or melanin, no suitable markers to determine the precise localization of melanin in the epidermis have been reported. In this study, we showed that melanocore-interacting Kif1c-tail (M-INK), a recently developed fluorescent probe for visualizing mature melanosomes, binds to purified melanin in vitro, and applied it for detecting melanin in human skin tissues. Frozen skin sections from different phototypes were co-stained for the hemagglutinin (HA)-tagged M-INK probe and markers of melanocytes or keratinocytes, and a wide distribution of melanin was observed in the epidermis. Analysis of the different skin phototypes indicated that the fluorescent signals of HA-M-INK correlated well with skin color. The reconstruction of three-dimensional images of epidermal sheets enabled us to observe the spatial distribution of melanin in the epidermis. Thus, the HA-M-INK probe is an ideal tool to individually visualize melanin (or melanosome) distribution in melanocytes and in keratinocytes in skin tissues.
Volume 21(22)
Published 2020-11-12
DOI 10.3390/ijms21228514
PII ijms21228514
PMID 33198186
PMC PMC7697890
MeSH Adolescent Adult Animals COS Cells Cells, Cultured Chlorocebus aethiops Epidermal Cells / metabolism Epidermis / metabolism Female Humans Hyperpigmentation / metabolism Keratinocytes / metabolism Melanins / metabolism* Melanocytes / metabolism Melanosomes / metabolism* Middle Aged Skin / metabolism* Skin Pigmentation / physiology
IF 4.556
Resource
DNA material pEF-HA-M-INK (RDB18649)