論文 - 詳細
| RRC ID | 63463 |
|---|---|
| 著者 | Cho B, Yoon SH, Lee D, Koranteng F, Tattikota SG, Cha N, Shin M, Do H, Hu Y, Oh SY, Lee D, Vipin Menon A, Moon SJ, Perrimon N, Nam JW, Shim J. |
| タイトル | Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila. |
| ジャーナル | Nat Commun |
| Abstract |
The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution. |
| 巻・号 | 11(1) |
| ページ | 4483 |
| 公開日 | 2020-9-8 |
| DOI | 10.1038/s41467-020-18135-y |
| PII | 10.1038/s41467-020-18135-y |
| PMID | 32900993 |
| PMC | PMC7479620 |
| MeSH | Animals Animals, Genetically Modified Cell Differentiation / genetics Cell Lineage / genetics Drosophila melanogaster / cytology* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Ectoparasitic Infestations / genetics Ectoparasitic Infestations / metabolism Ectoparasitic Infestations / pathology Gene Expression Profiling Hematopoiesis / genetics Hemocytes / cytology* Hemocytes / metabolism* Host-Parasite Interactions / genetics Host-Parasite Interactions / physiology Lymphoid Tissue / cytology Lymphoid Tissue / metabolism Lymphoid Tissue / parasitology RNA-Seq Single-Cell Analysis Transcriptome* Wasps / pathogenicity |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | Tep4-gal4 Men-gal4 |