論文 - 詳細
| RRC ID | 6349 |
|---|---|
| 著者 | Siripattarapravat K, Pinmee B, Venta PJ, Chang CC, Cibelli JB. |
| タイトル | Somatic cell nuclear transfer in zebrafish. |
| ジャーナル | Nat Methods |
| Abstract |
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones. |
| 巻・号 | 6(10) |
| ページ | 733-5 |
| 公開日 | 2009-10-1 |
| DOI | 10.1038/nmeth.1369 |
| PII | nmeth.1369 |
| PMID | 19718031 |
| MeSH | Animals Genetic Engineering / methods* Hybrid Cells / transplantation* Nuclear Transfer Techniques Zebrafish / anatomy & histology* Zebrafish / genetics* |
| IF | 30.822 |
| 引用数 | 26 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| リソース情報 | |
| ゼブラフィッシュ | HGn62A HGn28A HGn8E |