RRC ID 63495
Author Kameoka S, Kameyama T, Hayashi T, Sato S, Ohnishi N, Hayashi T, Murata-Kamiya N, Higashi H, Hatakeyama M, Takaoka A.
Title Helicobacter pylori induces IL-1β protein through the inflammasome activation in differentiated macrophagic cells.
Journal Biomed Res
Abstract More than 50% of people in the world are infected with Helicobacter pylori (H. pylori), which induces various gastric diseases. Especially, epidemiological studies have shown that H. pylori infection is a major risk factor for gastric cancer. It has been reported that the levels of interleukin (IL)-1β are upregulated in gastric tissues of patients with H. pylori infection. In this study, we investigated the induction mechanism of IL-1β during H. pylori infection. We found that IL-1βmRNA and protein were induced in phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells after H. pylori infection. This IL-1β production was inhibited by a caspase-1 inhibitor and a ROS inhibitor. Furthermore, K(+) efflux and Ca(2+) signaling were also involved in this process. These data suggest that NOD-like receptor (NLR) family, pyrin domain containing 3 (NLRP3) and its complex, known as NLRP3 inflammasome, are involved in IL-1β production during H. pylori infection because it is reported that NLRP3 inflammasome is activated by ROS, K(+) efflux and/or Ca(2+) signaling. These findings may provide therapeutic strategy for the control of gastric cancer in H. pylori-infected patients.
Volume 37(1)
Pages 21-7
Published 2016-1-1
DOI 10.2220/biomedres.37.21
PMID 26912137
MeSH Adenosine Triphosphate / metabolism Calcium Signaling Caspase 1 / metabolism Cell Line Extracellular Space / metabolism Helicobacter Infections / genetics Helicobacter Infections / immunology Helicobacter Infections / metabolism* Helicobacter pylori* / immunology Humans Inflammasomes / metabolism* Interleukin-1beta / genetics Interleukin-1beta / metabolism* Intracellular Space / metabolism Macrophages / immunology Macrophages / metabolism* Potassium / metabolism Reactive Oxygen Species / metabolism
IF 1.429
Human and Animal Cells THP-1(RCB1189)