RRC ID 63498
Author Usui M, Okamatsu Y, Sato T, Hanatani T, Moritani Y, Sano K, Yamamoto M, Nakashima K.
Title Thymus-expressed chemokine enhances Porphyromonas gingivalis LPS-induced osteoclast formation via NFATc1 activation.
Journal Arch Oral Biol
Abstract OBJECTIVE:P. gingivalis is a gram-negative anaerobic bacterium and a major periodontal pathogen. LPS produced by P. gingivalis promotes osteoclast formation. TECK is a CC chemokine whose expression is increased in gingival epithelial cells exposed to P. gingivalis LPS. In this study, we investigated the effect of TECK in osteoclastogenesis induced by P. gingivalis LPS.
DESIGNS:Real time reverse transcriptase polymerase chain reaction (RTPCR) analysis and western blotting were performed to confirm TECK in MG63, human osteoblast cell line and primary murine osteoblasts and CCR9 in RAW 264.7 cells and murine bone marrow macrophages (BMMs) as osteoclast precursors. P. gingivalis LPS-treated BMMs and Raw 264.7 cells were cultured with or without TECK or TECK antibody to examine the effect of TECK on osteoclast formation. Cocultures with murine osteoblasts and bone marrow cells were also treated with or without TECK or TECK antibody. Luciferase assay and western blotting were used to determine whether TECK-CCR9 induced osteoclastogenesis was mediated through NFATc1 or NF-kB signaling.
RESULTS:TECK was shown to be expressed by osteoblasts, and its receptor, CCR9, by osteoclast precursors. TECK increased P. gingivalis LPS-induced osteoclast numbers in an in vitro osteoclast formation assay using osteoclast precursors. The enhanced osteoclast formation by TECK was mediated by NFATc1, but not by NF-kB signaling.
CONCLUSION:TECK may be a novel regulator of osteoclast formation induced by P. gingivalis LPS in periodontitis.
Volume 66
Pages 77-85
Published 2016-6-1
DOI 10.1016/j.archoralbio.2016.02.011
PII S0003-9969(16)30038-3
PMID 26921718
MeSH Animals Bone Marrow Cells / drug effects Cell Differentiation / drug effects Cell Line Chemokines, CC / biosynthesis Chemokines, CC / pharmacology* Gingiva / cytology Gingiva / metabolism Humans Lipopolysaccharides / pharmacology* Macrophages / drug effects Mice Mice, Inbred BALB C NF-kappa B / metabolism NFATC Transcription Factors / metabolism* Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism Osteoclasts / cytology Osteoclasts / drug effects* Osteoclasts / metabolism Osteoclasts / microbiology Osteogenesis Porphyromonas gingivalis / drug effects Porphyromonas gingivalis / physiology* RAW 264.7 Cells Receptors, CCR / biosynthesis Recombinant Proteins / pharmacology Signal Transduction / drug effects
IF 1.931
Human and Animal Cells MG-63(RCB1890)