論文 - 詳細
| RRC ID | 63508 |
|---|---|
| 著者 | Zong R, Zhou T, Lin Z, Bao X, Xiu Y, Chen Y, Chen L, Ma JX, Liu Z, Zhou Y. |
| タイトル | Down-Regulation of MicroRNA-184 Is Associated With Corneal Neovascularization. |
| ジャーナル | Invest Ophthalmol Vis Sci |
| Abstract |
PURPOSE:Although microRNA-184 (miR-184) is abundantly expressed in the corneas, the role of miR-184 in corneal neovascularization remains unknown. Here we investigated the association between miR-184 expression and corneal neovascularization. METHODS:Quantitative real-time PCR assay was conducted to detect the expression of miR-184 and its potential target genes in the corneal epithelium of rats with corneal suture-induced neovascularization. MicroRNA-184 was also applied topically to the suture rats. Mimic and inhibitor of miR-184 were transfected into the cultured human umbilical vein endothelial cells (HUVECs), human corneal epithelial (HCE) cells, and simian choroidal endothelial cells (RF/6A). The following experiments were performed to evaluate the effects of miR-184 in these transfected cells: cell proliferation by cell viability assay, cell migration by a scratch wound test, VEGF-induced tube formation, and VEGF and β-catenin levels by Western blot analysis. RESULTS:The expression of miR-184 was significantly reduced, whereas the gene expression of frizzled-4, a receptor of the Wnt pathway, was up-regulated in the corneal epithelium of corneal suture rats. The corneal neovascularization induced by suture was ameliorated by topical administration of miR-184. In the cells transfected with mimic and inhibitor of miR-184, miR-184 significantly suppressed the cell proliferation and cell migration of HUVECs, miR-184 down-regulated VEGF, and β-catenin expression in HUVECs and HCE cells. Furthermore, miR-184 inhibited the tube formation of RF/6A cells. CONCLUSIONS:Down-regulation of miR-184 is associated with up-regulation of VEGF and Wnt/β-catenin expression as well as corneal neovascularization, indicating that miR-184 negatively regulates corneal neovascularization. |
| 巻・号 | 57(3) |
| ページ | 1398-407 |
| 公開日 | 2016-3-1 |
| DOI | 10.1167/iovs.15-17417 |
| PII | 2506767 |
| PMID | 27018842 |
| MeSH | Animals Blotting, Western Cell Movement Cell Proliferation Cell Survival Cells, Cultured Corneal Neovascularization / genetics* Corneal Neovascularization / metabolism Corneal Neovascularization / pathology Disease Models, Animal Down-Regulation* Humans Male MicroRNAs / biosynthesis MicroRNAs / genetics* RNA, Messenger / genetics* Rats Rats, Wistar Real-Time Polymerase Chain Reaction |
| IF | 3.47 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |