RRC ID 63510
Author Kaneko S.
Title In Vitro Generation of Antigen-Specific T Cells from Induced Pluripotent Stem Cells of Antigen-Specific T Cell Origin.
Journal Methods Mol Biol
Abstract Induced pluripotent stem (iPS) cells derived from T lymphocyte (T-iPS cells) preserve the T cell receptor (TCR) α and β gene rearrangements identical to the original T cell clone. Re-differentiated CD8 single positive αβ T cells from the T-iPS cells exhibited antigen-specific cytotoxicity, improved proliferative response, and elongation of telomere indicating rejuvenation of antigen specific T cell immunity in vitro. To regenerate antigen specific cytotoxic T lymphocytes (CTL), first, we have optimized a method for reprogramming-resistant CD8 T cell clones into T-iPS cells by using sendaiviral vectors. Second, we have optimized stepwise differentiation methods for inducing hematopoietic progenitor cells, T cell progenitors, and functionally matured CD8 single positive CTL. These protocols provide useful in vitro tools and models both for research of antigen-specific T cell immunotherapy and for research of normal and pathological thymopoiesis.
Volume 1393
Pages 67-73
Published 2016-1-1
DOI 10.1007/978-1-4939-3338-9_6
PMID 27033216
MeSH Animals CD8-Positive T-Lymphocytes / metabolism* Cell Differentiation Cells, Cultured Cellular Reprogramming* Cytotoxicity, Immunologic Humans Induced Pluripotent Stem Cells / physiology* Mice Receptors, Antigen, T-Cell, alpha-beta / metabolism T-Lymphocytes, Cytotoxic / metabolism*
Human and Animal Cells 10T1/2(RCB0247) OP9/DL1