RRC ID 6356
Author Fritz JA, Behm CA.
Title CUTI-1: A novel tetraspan protein involved in C. elegans CUTicle formation and epithelial integrity.
Journal PLoS ONE
Abstract The nematode cuticle is a tough extracellular matrix composed primarily of cross-linked collagens and non-collagenous cuticulins. It is required for nematode motility and protection from the external environment. Little is known about how the complex process of cuticle formation has been adapted to the specialized requirements of the nematode cuticle, which is structurally and compositionally unique from other organisms. The C. elegans gene cuti-1 (CUTicle and epithelial Integrity) encodes a nematode-specific protein. We have shown that CUTI-1 is expressed in the epithelia and in seam cells. Within these tissues the expression of cuti-1 mRNA cycles throughout development in line with the molting cycle, a process that involves synthesis of a new cuticle. In addition, knockdown of cuti-1 by RNA interference (RNAi) results in worms that display post-embryonic phenotypes related to cuticle dysfunction and defects in epithelial integrity. This is one of the first reports of a nematode-specific protein involved in extracellular matrix formation. It provides further insight into how novel ways have evolved to regulate the formation of the cuticle, which is the primary protective barrier and skeletal component of nematodes.
Volume 4(4)
Pages e5117
Published 2009
DOI 10.1371/journal.pone.0005117
PMID 19357781
PMC PMC2663847
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / anatomy & histology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Epithelium / metabolism* Epithelium / ultrastructure Extracellular Matrix / metabolism Genes, Reporter Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Two-Hybrid System Techniques Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism
IF 2.766
Times Cited 11
WOS Category MULTIDISCIPLINARY SCIENCES
Resource
C.elegans tm1388 tm2253