Reference - Detail
| RRC ID | 63662 |
|---|---|
| Author | Mitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguchi S, Norimine J. |
| Title | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells. |
| Journal | J Vet Med Sci |
| Abstract |
Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46+cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity. |
| Volume | 83(1) |
| Pages | 134-141 |
| Published | 2021-1-21 |
| DOI | 10.1292/jvms.20-0423 |
| PMID | 33177289 |
| PMC | PMC7870392 |
| MeSH | Animals Cattle Cytokines HEK293 Cells Humans Interleukin-2* / genetics Lymphocyte Activation* Recombinant Proteins / genetics T-Lymphocytes |
| IF | 1.049 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ILT-Mat(RCB0475) |