| RRC ID |
6370
|
| Author |
Han T, Manoharan AP, Harkins TT, Bouffard P, Fitzpatrick C, Chu DS, Thierry-Mieg D, Thierry-Mieg J, Kim JK.
|
| Title |
26G endo-siRNAs regulate spermatogenic and zygotic gene expression in Caenorhabditis elegans.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Endogenous small interfering RNAs (endo-siRNAs) regulate diverse gene expression programs in eukaryotes by either binding and cleaving mRNA targets or mediating heterochromatin formation; however, the mechanisms of endo-siRNA biogenesis, sorting, and target regulation remain poorly understood. Here we report the identification and function of a specific class of germline-generated endo-siRNAs in Caenorhabditis elegans that are 26 nt in length and contain a guanine at the first nucleotide position (i.e., 26G RNAs). 26G RNAs regulate gene expression during spermatogenesis and zygotic development, and their biogenesis requires the ERI-1 exonuclease and the RRF-3 RNA-dependent RNA polymerase (RdRP). Remarkably, we identified two nonoverlapping subclasses of 26G RNAs that sort into specific RNA-induced silencing complexes (RISCs) and differentially regulate distinct mRNA targets. Class I 26G RNAs target genes are expressed during spermatogenesis, whereas class II 26G RNAs are maternally inherited and silence gene expression during zygotic development. These findings implicate a class of endo-siRNAs in the global regulation of transcriptional programs required for fertility and development.
|
| Volume |
106(44)
|
| Pages |
18674-9
|
| Published |
2009-11-3
|
| DOI |
10.1073/pnas.0906378106
|
| PII |
0906378106
|
| PMID |
19846761
|
| PMC |
PMC2765456
|
| MeSH |
Animals
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / metabolism
Exoribonucleases / metabolism
Gene Expression Regulation, Developmental*
Gene Silencing
Germ Cells / metabolism
Guanine / metabolism*
Male
RNA, Helminth / classification
RNA, Helminth / metabolism
RNA, Small Interfering / biosynthesis
RNA, Small Interfering / classification
RNA, Small Interfering / metabolism*
Sequence Analysis, DNA
Spermatogenesis / genetics*
Zygote / metabolism*
|
| IF |
9.412
|
| Times Cited |
92
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| C.elegans |
tm1860
tm1155
tm1184 |
