論文 - 詳細
| RRC ID | 63743 |
|---|---|
| 著者 | Miyazaki T, Hanamatsu H, Xu L, Onodera T, Furukawa JI, Homan K, Baba R, Kawasaki T, Iwasaki N. |
| タイトル | Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Cartilage damage may eventually lead to osteoarthritis because it is difficult to repair. Human-induced pluripotent stem cell (iPSC)-derived chondrocytes may potentially be used to treat cartilage damage, but the tumorigenicity of iPSCs is a major concern for their application in regenerative medicine. Many glycoconjugates serve as stem cell markers, and glycosphingolipids (GSLs) including H type 1 antigen (Fucα1-2Galβ1-3GlcNAc) have been expressed on the surface of iPSCs. The purpose of the present study was to investigate whether GSL-glycome analysis is useful for quality control of residual iPSCs in chondrocytes. We performed GSL-glycome analysis of undifferentiated iPSCs in chondrocytes by combining glycoblotting and aminolysis-sialic acid linkage-specific alkylamidation (SALSA) method, enabling the detection of small quantities of iPSC-specific GSL-glycans from 5 × 104 cells. Furthermore, we estimated the residual amount of iPSCs using R-17F antibody, which possesses cytotoxic activity toward iPSCs that is dependent on the Lacto-N-fucopentaose I (LNFP I) of GSL. Moreover, we could detect a small number of LNFP I during mesenchymal stem cells (MSCs) differentiation from iPSCs. This is the first demonstration that GSL-glycome analysis is useful for detecting undifferentiated iPSCs, and can thereby support safe regenerative medicine. |
| 巻・号 | 21(1) |
| 公開日 | 2019-12-28 |
| DOI | 10.3390/ijms21010231 |
| PII | ijms21010231 |
| PMID | 31905707 |
| PMC | PMC6982033 |
| MeSH | Cell Differentiation* Cell Line Cells, Cultured Chondrocytes / cytology Chondrocytes / metabolism* Glycomics / methods Glycosphingolipids / metabolism* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Oligosaccharides / metabolism |
| IF | 4.556 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 606A1(HPS0328) |