RRC ID 6397
著者 Pontier DB, Tijsterman M.
タイトル A robust network of double-strand break repair pathways governs genome integrity during C. elegans development.
ジャーナル Curr Biol
Abstract To preserve genomic integrity, various mechanisms have evolved to repair DNA double-strand breaks (DSBs). Depending on cell type or cell cycle phase, DSBs can be repaired error-free, by homologous recombination, or with concomitant loss of sequence information, via nonhomologous end-joining (NHEJ) or single-strand annealing (SSA). Here, we created a transgenic reporter system in C. elegans to investigate the relative contribution of these pathways in somatic cells during animal development. Although all three canonical pathways contribute to repair in the soma, in their combined absence, animals develop without growth delay and chromosomal breaks are still efficiently repaired. This residual repair, which we call alternative end-joining, dominates DSB repair only in the absence of NHEJ and resembles SSA, but acts independent of the SSA nuclease XPF and repair proteins from other pathways. The dynamic interplay between repair pathways might be developmentally regulated, because it was lost from terminally differentiated cells in adult animals. Our results demonstrate profound versatility in DSB repair pathways for somatic cells of C. elegans, which are thus extremely fit to deal with chromosomal breaks.
巻・号 19(16)
ページ 1384-8
公開日 2009-8-25
DOI 10.1016/j.cub.2009.06.045
PII S0960-9822(09)01322-0
PMID 19646877
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics DNA Breaks, Double-Stranded* DNA Repair / genetics DNA Repair / physiology* DNA, Helminth / genetics* DNA, Helminth / metabolism Genes, Reporter Genome, Helminth Larva Models, Genetic Polymerase Chain Reaction Recombinant Fusion Proteins / physiology Sequence Deletion Transgenes / genetics
IF 9.601
引用数 25
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1145 tm2073