| RRC ID |
64011
|
| 著者 |
Hong Zheng, Xiao Lu, Kai Li, Feng Zhu, Chenhao Zhao, Taiping Liu, Yan Ding, Yong Fu, Kun Zhang, Taoli Zhou, Jigang Dai, Yuzhang Wu, Wenyue Xu
|
| タイトル |
ATGs ubiquitination is required for circumsporozoite protein to subvert host innate immunity against malaria liver stage
|
| Abstract |
Although exoerythrocytic forms (EEFs) of liver stage malaria parasite in parasitophorous vacuole (PV) encountered with robust host innate immunity, EEFs can still survive and successfully complete infection of hepatocytes, and the underlying mechanism is largely unknown. Here, we showed that sporozoite circumsporozoite protein (CSP) translocated from the parasitophorous vacuole into the hepatocyte cytoplasm significantly inhibited the killing of exo-erythrocytic forms (EEFs) by interferon-gamma (IFN-γ). Attenuation of IFN-γ-mediated killing of EEFs by CSP was dependent on its ability to reduce the levels of autophagy-related genes (ATGs) in hepatocytes. The ATGs downregulation occurred through its enhanced ubiquitination mediated by E3 ligase NEDD4, an enzyme that was upregulated by CSP when it translocated from the cytoplasm into the nucleus of hepatocytes via its nuclear localization signal (NLS) domain. Thus, we have revealed an unrecognized role of CSP in subverting host innate immunity and shed new light for a prophylaxis strategy against liver-stage infection.
|
| DOI |
10.1101/2021.01.20.427456
|
| PMID |
35222391
|
| PMC |
PMC8864237
|
| MeSH |
Animals
Immunity, Innate
Interferon-gamma / metabolism
Liver / metabolism
Malaria*
Protozoan Proteins
Rodentia* / metabolism
Ubiquitination
|
| リソース情報 |
| 実験動物マウス |
RBRC02975 |
