| RRC ID |
64148
|
| 著者 |
Kajitani K, Honda K, Terada H, Yasui T, Sumi T, Koyama M, Ishiko O.
|
| タイトル |
Human Papillomavirus E6 Knockdown Restores Adenovirus Mediated-estrogen Response Element Linked p53 Gene Transfer in HeLa Cells.
|
| ジャーナル |
Asian Pac J Cancer Prev
|
| Abstract |
The p53 gene is inactivated by the human papillomavirus (HPV) E6 protein in the majority of cervical cancers. Treatment of HeLa S3 cells with siRNA for HPV E6 permitted adenovirus-mediated transduction of a p53 gene linked to an upstream estrogen response element (ERE). Our previous study in non-siRNA treated HHUA cells, which are derived from an endometrial cancer and express estrogen receptor β, showed enhancing effects of an upstream ERE on adenovirus-mediated p53 gene transduction. In HeLa S3 cells treated with siRNA for HPV E6, adenovirus-mediated transduction was enhanced by an upstream ERE linked to a p53 gene carrying a proline variant at codon 72, but not for a p53 gene with arginine variant at codon 72. Expression levels of p53 mRNA and Coxsackie/adenovirus receptor (CAR) mRNA after adenovirus-mediated transfer of an ERE-linked p53 gene (proline variant at codon 72) were higher compared with those after non-ERE-linked p53 gene transfer in siRNA-treated HeLa S3 cells. Western blot analysis showed lower β-tubulin levels and comparatively higher p53/β-tubulin or CAR /β-tubulin ratios in siRNA-treated HeLa S3 cells after adenovirus-mediated ERE-linked p53 gene (proline variant at codon 72) transfer compared with those in non-siRNA-treated cells. Apoptosis, as measured by annexin V binding, was higher after adenovirus-mediated ERE-linked p53 gene (proline variant at codon 72) transfer compared with that after non-ERE-linked p53 gene transfer in siRNA-treated cells.
|
| 巻・号 |
16(18)
|
| ページ |
8239-45
|
| 公開日 |
2015-1-1
|
| DOI |
10.7314/apjcp.2015.16.18.8239
|
| PMID |
26745067
|
| MeSH |
Adenoviridae / genetics*
Blotting, Western
DNA-Binding Proteins / antagonists & inhibitors*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Estrogens / pharmacology*
Female
Flow Cytometry
Genetic Therapy*
HeLa Cells
Humans
Oncogene Proteins, Viral / antagonists & inhibitors*
Oncogene Proteins, Viral / genetics
Oncogene Proteins, Viral / metabolism
RNA, Messenger / genetics
RNA, Small Interfering / genetics
Real-Time Polymerase Chain Reaction
Response Elements / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Protein p53 / administration & dosage*
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism
Uterine Cervical Neoplasms / genetics
Uterine Cervical Neoplasms / therapy*
Uterine Cervical Neoplasms / virology
|
| リソース情報 |
| ヒト・動物細胞 |
HHUA(RCB0658) |
