論文 - 詳細
| RRC ID | 64148 |
|---|---|
| 著者 | Kajitani K, Honda K, Terada H, Yasui T, Sumi T, Koyama M, Ishiko O. |
| タイトル | Human Papillomavirus E6 Knockdown Restores Adenovirus Mediated-estrogen Response Element Linked p53 Gene Transfer in HeLa Cells. |
| ジャーナル | Asian Pac J Cancer Prev |
| Abstract |
The p53 gene is inactivated by the human papillomavirus (HPV) E6 protein in the majority of cervical cancers. Treatment of HeLa S3 cells with siRNA for HPV E6 permitted adenovirus-mediated transduction of a p53 gene linked to an upstream estrogen response element (ERE). Our previous study in non-siRNA treated HHUA cells, which are derived from an endometrial cancer and express estrogen receptor β, showed enhancing effects of an upstream ERE on adenovirus-mediated p53 gene transduction. In HeLa S3 cells treated with siRNA for HPV E6, adenovirus-mediated transduction was enhanced by an upstream ERE linked to a p53 gene carrying a proline variant at codon 72, but not for a p53 gene with arginine variant at codon 72. Expression levels of p53 mRNA and Coxsackie/adenovirus receptor (CAR) mRNA after adenovirus-mediated transfer of an ERE-linked p53 gene (proline variant at codon 72) were higher compared with those after non-ERE-linked p53 gene transfer in siRNA-treated HeLa S3 cells. Western blot analysis showed lower β-tubulin levels and comparatively higher p53/β-tubulin or CAR /β-tubulin ratios in siRNA-treated HeLa S3 cells after adenovirus-mediated ERE-linked p53 gene (proline variant at codon 72) transfer compared with those in non-siRNA-treated cells. Apoptosis, as measured by annexin V binding, was higher after adenovirus-mediated ERE-linked p53 gene (proline variant at codon 72) transfer compared with that after non-ERE-linked p53 gene transfer in siRNA-treated cells. |
| 巻・号 | 16(18) |
| ページ | 8239-45 |
| 公開日 | 2015-1-1 |
| DOI | 10.7314/apjcp.2015.16.18.8239 |
| PMID | 26745067 |
| MeSH | Adenoviridae / genetics* Blotting, Western DNA-Binding Proteins / antagonists & inhibitors* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Estrogens / pharmacology* Female Flow Cytometry Genetic Therapy* HeLa Cells Humans Oncogene Proteins, Viral / antagonists & inhibitors* Oncogene Proteins, Viral / genetics Oncogene Proteins, Viral / metabolism RNA, Messenger / genetics RNA, Small Interfering / genetics Real-Time Polymerase Chain Reaction Response Elements / genetics* Reverse Transcriptase Polymerase Chain Reaction Tumor Suppressor Protein p53 / administration & dosage* Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism Uterine Cervical Neoplasms / genetics Uterine Cervical Neoplasms / therapy* Uterine Cervical Neoplasms / virology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HHUA(RCB0658) |