Reference - Detail
| RRC ID | 64245 |
|---|---|
| Author | Kilchert C, Wittmann S, Passoni M, Shah S, Granneman S, Vasiljeva L. |
| Title | Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1. |
| Journal | Cell Rep |
| Abstract |
In eukaryotic cells, inefficient splicing is surprisingly common and leads to the degradation of transcripts with retained introns. How pre-mRNAs are committed to nuclear decay is unknown. Here, we uncover a mechanism by which specific intron-containing transcripts are targeted for nuclear degradation in fission yeast. Sequence elements within these "decay-promoting" introns co-transcriptionally recruit the exosome specificity factor Mmi1, which induces degradation of the unspliced precursor and leads to a reduction in the levels of the spliced mRNA. This mechanism negatively regulates levels of the RNA helicase DDX5/Dbp2 to promote cell survival in response to stress. In contrast, fast removal of decay-promoting introns by co-transcriptional splicing precludes Mmi1 recruitment and relieves negative expression regulation. We propose that decay-promoting introns facilitate the regulation of gene expression. Based on the identification of multiple additional Mmi1 targets, including mRNAs, long non-coding RNAs, and sn/snoRNAs, we suggest a general role in RNA regulation for Mmi1 through transcript degradation. |
| Volume | 13(11) |
| Pages | 2504-2515 |
| Published | 2015-12-22 |
| DOI | 10.1016/j.celrep.2015.11.026 |
| PII | S2211-1247(15)01326-1 |
| PMID | 26670050 |
| PMC | PMC4695336 |
| MeSH | Base Sequence Chromatin Immunoprecipitation DEAD-box RNA Helicases / metabolism Exosomes / metabolism* Gene Expression Regulation, Fungal Introns Protein Binding RNA Precursors / metabolism RNA Splicing RNA Stability RNA, Messenger / metabolism* RNA, Untranslated / metabolism Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Analysis, RNA Transcriptome mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism* |
| IF | 8.109 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | FY23475 FY9118 |