論文 - 詳細
| RRC ID | 64249 |
|---|---|
| 著者 | Masuda K, Renard-Guillet C, Shirahige K, Sutani T. |
| タイトル | Bioinformatical dissection of fission yeast DNA replication origins. |
| ジャーナル | Open Biol |
| Abstract |
Replication origins in eukaryotes form a base for assembly of the pre-replication complex (pre-RC), thereby serving as an initiation site of DNA replication. Characteristics of replication origin vary among species. In fission yeast Schizosaccharomyces pombe, DNA of high AT content is a distinct feature of replication origins; however, it remains to be understood what the general molecular architecture of fission yeast origin is. Here, we performed ChIP-seq mapping of Orc4 and Mcm2, two representative components of the pre-RC, and described the characteristics of their binding sites. The analysis revealed that fission yeast efficient origins are associated with two similar but independent features: a ≥15 bp-long motif with stretches of As and an AT-rich region of a few hundred bp. The A-rich motif was correlated with chromosomal binding of Orc, a DNA-binding component in the pre-RC, whereas the AT-rich region was associated with efficient binding of the DNA replicative helicase Mcm. These two features, in combination with the third feature, a transcription-poor region of approximately 1 kb, enabled to distinguish efficient replication origins from the rest of chromosome arms with high accuracy. This study, hence, provides a model that describes how multiple functional elements specify DNA replication origins in fission yeast genome. |
| 巻・号 | 10(7) |
| ページ | 200052 |
| 公開日 | 2020-7-1 |
| DOI | 10.1098/rsob.200052 |
| PMID | 32692956 |
| PMC | PMC7574548 |
| MeSH | Cell Cycle / genetics Cell Cycle Proteins / genetics* DNA Helicases / genetics DNA Replication / genetics* DNA-Binding Proteins / genetics Genome, Fungal / genetics Origin Recognition Complex / genetics* Replication Origin / genetics* Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics* |
| IF | 4.931 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY20933 FY8155 |