RRC ID 64249
著者 Masuda K, Renard-Guillet C, Shirahige K, Sutani T.
タイトル Bioinformatical dissection of fission yeast DNA replication origins.
ジャーナル Open Biol
Abstract Replication origins in eukaryotes form a base for assembly of the pre-replication complex (pre-RC), thereby serving as an initiation site of DNA replication. Characteristics of replication origin vary among species. In fission yeast Schizosaccharomyces pombe, DNA of high AT content is a distinct feature of replication origins; however, it remains to be understood what the general molecular architecture of fission yeast origin is. Here, we performed ChIP-seq mapping of Orc4 and Mcm2, two representative components of the pre-RC, and described the characteristics of their binding sites. The analysis revealed that fission yeast efficient origins are associated with two similar but independent features: a ≥15 bp-long motif with stretches of As and an AT-rich region of a few hundred bp. The A-rich motif was correlated with chromosomal binding of Orc, a DNA-binding component in the pre-RC, whereas the AT-rich region was associated with efficient binding of the DNA replicative helicase Mcm. These two features, in combination with the third feature, a transcription-poor region of approximately 1 kb, enabled to distinguish efficient replication origins from the rest of chromosome arms with high accuracy. This study, hence, provides a model that describes how multiple functional elements specify DNA replication origins in fission yeast genome.
巻・号 10(7)
ページ 200052
公開日 2020-7-1
DOI 10.1098/rsob.200052
PMID 32692956
PMC PMC7574548
MeSH Cell Cycle / genetics Cell Cycle Proteins / genetics* DNA Helicases / genetics DNA Replication / genetics* DNA-Binding Proteins / genetics Genome, Fungal / genetics Origin Recognition Complex / genetics* Replication Origin / genetics* Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics*
IF 4.931
リソース情報
酵母 FY20933 FY8155