論文 - 詳細
| RRC ID | 64272 |
|---|---|
| 著者 | Ichikawa K, Tomioka S, Suzuki Y, Nakamura R, Doi K, Yoshimura J, Kumagai M, Inoue Y, Uchida Y, Irie N, Takeda H, Morishita S. |
| タイトル | Centromere evolution and CpG methylation during vertebrate speciation. |
| ジャーナル | Nat Commun |
| Abstract |
Centromeres and large-scale structural variants evolve and contribute to genome diversity during vertebrate speciation. Here, we perform de novo long-read genome assembly of three inbred medaka strains that are derived from geographically isolated subpopulations and undergo speciation. Using single-molecule real-time (SMRT) sequencing, we obtain three chromosome-mapped genomes of length ~734, ~678, and ~744Mbp with a resource of twenty-two centromeric regions of length 20-345kbp. Centromeres are positionally conserved among the three strains and even between four pairs of chromosomes that were duplicated by the teleost-specific whole-genome duplication 320-350 million years ago. The centromeres do not all evolve at a similar pace; rather, centromeric monomers in non-acrocentric chromosomes evolve significantly faster than those in acrocentric chromosomes. Using methylation sensitive SMRT reads, we uncover centromeres are mostly hypermethylated but have hypomethylated sub-regions that acquire unique sequence compositions independently. These findings reveal the potential of non-acrocentric centromere evolution to contribute to speciation. |
| 巻・号 | 8(1) |
| ページ | 1833 |
| 公開日 | 2017-11-28 |
| DOI | 10.1038/s41467-017-01982-7 |
| PII | 10.1038/s41467-017-01982-7 |
| PMID | 29184138 |
| PMC | PMC5705604 |
| MeSH | Animals Base Sequence Centromere / classification Centromere / genetics* Chromosome Mapping CpG Islands* Evolution, Molecular Genetic Association Studies Genetic Markers Genetic Speciation* Genetic Variation Genome Methylation Models, Theoretical Molecular Sequence Annotation Mutation Oryzias / genetics Phylogeny Polymorphism, Single Nucleotide Telomere / genetics Vertebrates / genetics* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 27 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 28.0 |
| リソース情報 | |
| メダカ | HdrR-II1 (IB178) HNI-II (IB176) HSOK (IB183) |