論文 - 詳細
| RRC ID | 64277 |
|---|---|
| 著者 | Yokosawa T, Sato A, Wakasugi K. |
| タイトル | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells. |
| ジャーナル | Genes (Basel) |
| Abstract |
The novel high-affinity tryptophan (Trp)-selective transport system is present at elevated levels in human interferon-γ (IFN-γ)-treated and indoleamine 2,3-dioxygenase 1 (IDO1)-expressing cells. High-affinity Trp uptake into cells results in extracellular Trp depletion and immune suppression. We have previously shown that both IDO1 and tryptophanyl-tRNA synthetase (TrpRS), whose expression levels are increased by IFN-γ, have a crucial function in high-affinity Trp uptake into human cells. Here, we aimed to elucidate the relationship between TrpRS and IDO1 in high-affinity Trp uptake. We demonstrated that overexpression of IDO1 in HeLa cells drastically enhances high-affinity Trp uptake upon addition of purified TrpRS protein to uptake assay buffer. We also clarified that high-affinity Trp uptake by Trp-starved cells is significantly enhanced by the addition of TrpRS protein to the assay buffer. Moreover, we showed that high-affinity Trp uptake is also markedly elevated by the addition of TrpRS protein to the assay buffer of cells overexpressing another Trp-metabolizing enzyme, tryptophan 2,3-dioxygenase (TDO2). Taken together, we conclude that Trp deficiency is crucial for high-affinity Trp uptake mediated by extracellular TrpRS. |
| 巻・号 | 11(12) |
| 公開日 | 2020-11-27 |
| DOI | 10.3390/genes11121423 |
| PII | genes11121423 |
| PMID | 33261077 |
| PMC | PMC7760169 |
| MeSH | Biological Transport / drug effects Buffers Culture Media HeLa Cells Humans Indoleamine-Pyrrole 2,3,-Dioxygenase / genetics Indoleamine-Pyrrole 2,3,-Dioxygenase / physiology Interferon-gamma / pharmacology Interferon-gamma / physiology Mutation, Missense Point Mutation Recombinant Proteins / metabolism Transfer RNA Aminoacylation Tryptophan / deficiency* Tryptophan / metabolism Tryptophan Oxygenase / metabolism Tryptophan-tRNA Ligase / pharmacology Tryptophan-tRNA Ligase / physiology* Up-Regulation |
| IF | 3.759 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genome Network Project Human cDNA Clone IRAK049I14 (HGX019806) |
| ヒト・動物細胞 | HeLa(RCB0007) |