論文 - 詳細
| RRC ID | 64301 |
|---|---|
| 著者 | Abe K, Kanehira M, Ohkouchi S, Kumata S, Suzuki Y, Oishi H, Noda M, Sakurada A, Miyauchi E, Fujiwara T, Harigae H, Okada Y. |
| タイトル | Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer. |
| ジャーナル | Cancer Med |
| Abstract |
Lung cancer is the most common cause of cancer-related death in developed countries; therefore, the generation of effective targeted therapeutic regimens is essential. Recently, gene therapy approaches toward malignant cells have emerged as attractive molecular therapeutics. Previous studies have indicated that stanniocalcin-1 (STC-1), a hormone involved in calcium and phosphate homeostasis, positively regulates proliferation, apoptosis resistance, and glucose metabolism in lung cancer cell lines. In this study, we investigated if targeting STC-1 in tumor cells could be a promising strategy for lung cancer gene therapy. We confirmed that STC-1 levels in peripheral blood were higher in lung cancer patients than in healthy donors and that STC-1 expression was observed in five out of eight lung cancer cell lines. A vector expressing a suicide gene, uracil phosphoribosyltransferase (UPRT), under the control of the STC-1 promoter, was constructed (pPSTC-1 -UPRT) and transfected into three STC-1-positive cell lines, PC-9, A549, and H1299. When stably transfected, we observed significant cell growth inhibition using 5-fluorouracil (5-FU) treatment. Furthermore, growth of the STC-1-negative lung cancer cell line, LK-2 was significantly arrested when combined with STC-1-positive cells transfected with pPSTC-1 -UPRT. We believe that conferring cytotoxicity in STC-1-positive lung cancer cells using a suicide gene may be a useful therapeutic strategy for lung cancer. |
| 巻・号 | 10(9) |
| ページ | 3085-3100 |
| 公開日 | 2021-5-1 |
| DOI | 10.1002/cam4.3852 |
| PMID | 33826244 |
| PMC | PMC8085941 |
| MeSH | A549 Cells Animals Antimetabolites, Antineoplastic / therapeutic use Apoptosis Cell Line, Tumor Cell Proliferation Disease Models, Animal Female Fluorouracil / therapeutic use Genes, Reporter Genes, Transgenic, Suicide Genetic Therapy / methods* Glucose / metabolism Glycoproteins / blood Glycoproteins / genetics Glycoproteins / metabolism* Humans Luciferases / genetics Luciferases / metabolism Lung Neoplasms / blood Lung Neoplasms / metabolism Lung Neoplasms / pathology Lung Neoplasms / therapy* Mice Mice, Inbred BALB C Mice, Nude Molecular Targeted Therapy / methods* Pentosyltransferases / genetics Pentosyltransferases / metabolism* Plasmids Promoter Regions, Genetic / physiology Reverse Transcriptase Polymerase Chain Reaction Telomerase / genetics Transfection Xenograft Model Antitumor Assays |
| IF | 3.491 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LC-1/sq(RCB0455) LC-2/ad(RCB0440) LK-2(RCB1970) PC-9(RCB4455) |