論文 - 詳細
| RRC ID | 64354 |
|---|---|
| 著者 | Wu J, Li X, Gao Z, Pang L, Liu X, Huang X, Wang Y, Wang Z. |
| タイトル | RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis. |
| ジャーナル | Hum Mol Genet |
| Abstract |
CLP1, TSEN complex, and VCP are evolutionarily conserved proteins whose mutations are associated with neurodegenerative diseases. In this study, we have found that they are also involved in germline differentiation. To optimize both quantity and quality in gametes production, germ cells expand themselves through limited mitotic cycles prior to meiosis. Stemming from our previous findings on the correlation between mRNA 3'-processing and meiosis entry, here we identify that the RNA kinase Cbc, the Drosophila member of the highly conserved CLP1 family, is a component of the program regulating the transition from mitosis to meiosis. Using genetic manipulations in Drosophila testis, we demonstrate that nuclear Cbc is required to promote meiosis entry. Combining biochemical and genetic methods, we reveal that Cbc physically and/or genetically intersects with Tsen54 and TER94 (VCP ortholog) in this process. The C-terminal half of Tsen54 is both necessary and sufficient for its binding with Cbc. Further, we illustrate the functional conservation between Cbc and mammalian CLP1 in the assays of subcellular localization and Drosophila fertility. As CLP1, TSEN complex, and VCP have also been identified in neurodegenerations of animal models, a mechanism involving these factors seems to be shared in gametogenesis and neurogenesis. |
| 巻・号 | 30(17) |
| ページ | 1569-1578 |
| 公開日 | 2021-8-12 |
| DOI | 10.1093/hmg/ddab107 |
| PII | 6230813 |
| PMID | 33864361 |
| PMC | PMC8369837 |
| MeSH | Animals Animals, Genetically Modified Cell Differentiation / genetics Drosophila melanogaster / metabolism Gene Expression / genetics Gene Expression Regulation, Developmental / genetics Germ Cells / metabolism Male Meiosis / genetics* Meiosis / physiology Mutation Nuclear Proteins / metabolism* Phosphotransferases / metabolism* RNA / genetics Spermatogenesis / genetics* Spermatogenesis / physiology Testis / embryology Testis / metabolism Transcription Factors / metabolism* |
| IF | 5.101 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 5970R-3 |