| RRC ID |
64394
|
| 著者 |
Toyotome T, Arai T, Watanabe A, Kamei K.
|
| タイトル |
Detection of Substitutions at 98th, 121st, and 289th Amino Acid Residues in Cyp51A using Cycling Probes.
|
| ジャーナル |
Med Mycol J
|
| Abstract |
Azole-resistant Aspergillus fumigatus containing unique mutation(s) of cyp51A with tandem repeats in the promoter region has emerged and has become dispersed in environments worldwide. For this study, we designed primers and cycling probes to detect mutations associated with tandem repeats. Substitutions at the 293rd nucleotide (leucine or histidine at the 98th amino acid residue), at the 362nd nucleotide (tyrosine or phenylalanine at the 121st amino acid residue), and at the 865th nucleotide (threonine or alanine at the 289th amino acid residue) in cyp51A were detected using these primers and probes. These results suggest that the primer and probe sets are helpful in detecting these mutations and in differentiating the types of tandem repeats in cyp51A.
|
| 巻・号 |
61(1)
|
| ページ |
7-10
|
| 公開日 |
2020-1-1
|
| DOI |
10.3314/mmj.19-00014
|
| PMID |
32115445
|
| MeSH |
Amino Acids*
Aspergillus fumigatus / genetics*
Cytochrome P-450 Enzyme System*
Drug Resistance, Fungal
Fungal Proteins*
Mutation
Nucleotides
Phosphatidylcholines
Promoter Regions, Genetic
Tandem Repeat Sequences
|
| リソース情報 |
| 病原真核微生物 |
IFM 63432, 64732 (= OKH50) |
