RRC ID 64508
著者 Grmai L, Harsh S, Lu S, Korman A, Deb IB, Bach EA.
タイトル Transcriptomic analysis of feminizing somatic stem cells in the Drosophila testis reveals putative downstream effectors of the transcription factor Chinmo.
ジャーナル G3 (Bethesda)
Abstract One of the best examples of sexual dimorphism is the development and function of the gonads, ovaries and testes, which produce sex-specific gametes, oocytes, and spermatids, respectively. The development of these specialized germ cells requires sex-matched somatic support cells. The sexual identity of somatic gonadal cells is specified during development and must be actively maintained during adulthood. We previously showed that the transcription factor Chinmo is required to ensure the male sexual identity of somatic support cells in the Drosophila melanogaster testis. Loss of chinmo from male somatic gonadal cells results in feminization: they transform from squamous to epithelial-like cells that resemble somatic cells in the female gonad but fail to properly ensheath the male germline, causing infertility. To identify potential target genes of Chinmo, we purified somatic cells deficient for chinmo from the adult Drosophila testis and performed next-generation sequencing to compare their transcriptome to that of control somatic cells. Bioinformatics revealed 304 and 1549 differentially upregulated and downregulated genes, respectively, upon loss of chinmo in early somatic cells. Using a combination of methods, we validated several differentially expressed genes. These data sets will be useful resources to the community.
巻・号 11(4)
公開日 2021-4-15
DOI 10.1093/g3journal/jkab067
PII 6159614
PMID 33751104
PMC PMC8759813
MeSH Adult Stem Cells* / metabolism Animals Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Female Gonads / metabolism Male Nerve Tissue Proteins / genetics Testis / metabolism Transcription Factors / genetics Transcriptome
IF 2.781
リソース情報
ショウジョウバエ DGRC#104055