論文 - 詳細
| RRC ID | 64509 |
|---|---|
| 著者 | Ariss MM, Terry AR, Islam ABMMK, Hay N, Frolov MV. |
| タイトル | Amalgam regulates the receptor tyrosine kinase pathway through Sprouty in glial cell development in the Drosophila larval brain. |
| ジャーナル | J Cell Sci |
| Abstract |
The receptor tyrosine kinase (RTK) pathway plays an essential role in development and disease by controlling cell proliferation and differentiation. Here, we profile the Drosophila larval brain by single-cell RNA-sequencing and identify Amalgam (Ama), which encodes a cell adhesion protein of the immunoglobulin IgLON family, as regulating the RTK pathway activity during glial cell development. Depletion of Ama reduces cell proliferation, affects glial cell type composition and disrupts the blood-brain barrier (BBB), which leads to hemocyte infiltration and neuronal death. We show that Ama depletion lowers RTK activity by upregulating Sprouty (Sty), a negative regulator of the RTK pathway. Knockdown of Ama blocks oncogenic RTK signaling activation in the Drosophila glioma model and halts malignant transformation. Finally, knockdown of a human ortholog of Ama, LSAMP, results in upregulation of SPROUTY2 in glioblastoma cell lines, suggesting that the relationship between Ama and Sty is conserved. |
| 巻・号 | 133(19) |
| 公開日 | 2020-10-1 |
| DOI | 10.1242/jcs.250837 |
| PII | jcs.250837 |
| PMID | 32878945 |
| PMC | PMC7541346 |
| MeSH | Animals Brain / metabolism Drosophila* / metabolism Drosophila Proteins / genetics* Gene Expression Regulation, Developmental Immunoglobulins / genetics* Larva / metabolism Membrane Proteins / genetics* Membrane Proteins / metabolism Neuroglia / metabolism Receptor Protein-Tyrosine Kinases / genetics Receptor Protein-Tyrosine Kinases / metabolism |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#103970 |