論文 - 詳細
| RRC ID | 64513 |
|---|---|
| 著者 | Ma Z, Freeman MR. |
| タイトル | TrpML-mediated astrocyte microdomain Ca2+ transients regulate astrocyte-tracheal interactions. |
| ジャーナル | Elife |
| Abstract |
Astrocytes exhibit spatially-restricted near-membrane microdomain Ca2+transients in their fine processes. How these transients are generated and regulate brain function in vivo remains unclear. Here we show that Drosophila astrocytes exhibit spontaneous, activity-independent microdomain Ca2+ transients in their fine processes. Astrocyte microdomain Ca2+ transients are mediated by the TRP channel TrpML, stimulated by reactive oxygen species (ROS), and can be enhanced in frequency by the neurotransmitter tyramine via the TyrRII receptor. Interestingly, many astrocyte microdomain Ca2+ transients are closely associated with tracheal elements, which dynamically extend filopodia throughout the central nervous system (CNS) to deliver O2 and regulate gas exchange. Many astrocyte microdomain Ca2+ transients are spatio-temporally correlated with the initiation of tracheal filopodial retraction. Loss of TrpML leads to increased tracheal filopodial numbers, growth, and increased CNS ROS. We propose that local ROS production can activate astrocyte microdomain Ca2+ transients through TrpML, and that a subset of these microdomain transients promotes tracheal filopodial retraction and in turn modulate CNS gas exchange. |
| 巻・号 | 9 |
| 公開日 | 2020-12-7 |
| DOI | 10.7554/eLife.58952 |
| PII | 58952 |
| PMID | 33284108 |
| PMC | PMC7721441 |
| MeSH | Acetylcholine / pharmacology Action Potentials / physiology Animals Astrocytes / metabolism* Calcium / metabolism* Calcium Signaling / physiology Central Nervous System Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Enzyme Inhibitors / pharmacology Gene Expression Regulation / drug effects Gene Expression Regulation / physiology Lanthanum / pharmacology Membrane Microdomains / physiology* Mutation Reactive Oxygen Species Receptors, Biogenic Amine / genetics Receptors, Biogenic Amine / metabolism Tetrodotoxin / pharmacology Trachea / physiology* Transient Receptor Potential Channels / genetics Transient Receptor Potential Channels / metabolism* Tyramine / pharmacology gamma-Aminobutyric Acid / pharmacology |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109038 |