RRC ID 64517
Author Kim M, Du OY, Whitney RJ, Wilk R, Hu J, Krause HM, Kavaler J, Reed BH.
Title A Functional Analysis of the Drosophila Gene hindsight: Evidence for Positive Regulation of EGFR Signaling.
Journal G3 (Bethesda)
Abstract We have investigated the relationship between the function of the gene hindsight (hnt), which is the Drosophila homolog of Ras Responsive Element Binding protein-1 (RREB-1), and the EGFR signaling pathway. We report that hnt mutant embryos are defective in EGFR signaling dependent processes, namely chordotonal organ recruitment and oenocyte specification. We also show the temperature sensitive hypomorphic allele hntpebbled is enhanced by the hypomorphic MAPK allele rolled (rl1 ). We find that hnt overexpression results in ectopic DPax2 expression within the embryonic peripheral nervous system, and we show that this effect is EGFR-dependent. Finally, we show that the canonical U-shaped embryonic lethal phenotype of hnt, which is associated with premature degeneration of the extraembyonic amnioserosa and a failure in germ band retraction, is rescued by expression of several components of the EGFR signaling pathway (sSpi, Ras85DV12 , pntP1 ) as well as the caspase inhibitor p35 Based on this collection of corroborating evidence, we suggest that an overarching function of hnt involves the positive regulation of EGFR signaling.
Volume 10(1)
Pages 117-127
Published 2020-1-7
DOI 10.1534/g3.119.400829
PII g3.119.400829
PMID 31649045
PMC PMC6945037
MeSH Animals Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster ErbB Receptors / metabolism* Extracellular Signal-Regulated MAP Kinases / genetics Extracellular Signal-Regulated MAP Kinases / metabolism Germ Layers / embryology Germ Layers / metabolism Nuclear Proteins / genetics* Nuclear Proteins / metabolism PAX2 Transcription Factor / genetics PAX2 Transcription Factor / metabolism Signal Transduction* Transcription Factors / genetics* Transcription Factors / metabolism
IF 2.781