Reference - Detail
RRC ID | 6452 |
---|---|
Author | Ideo H, Fukushima K, Gengyo-Ando K, Mitani S, Dejima K, Nomura K, Yamashita K. |
Title | A Caenorhabditis elegans glycolipid-binding galectin functions in host defense against bacterial infection. |
Journal | J Biol Chem |
Abstract |
Galectins are a family of beta-galactoside-binding proteins that are widely found among animal species and that regulate diverse biological phenomena. To study the biological function of glycolipid-binding galectins, we purified recombinant Caenorhabditis elegans galectins (LEC-1-11) and studied their binding to C. elegans glycolipids. We found that LEC-8 binds to glycolipids in C. elegans through carbohydrate recognition. It has been reported that Cry5B-producing Bacillus thuringiensis strains can infect C. elegans and that the C. elegans Cry5B receptor molecules are glycolipids. We found that Cry5B and LEC-8 bound to C. elegans glycolipid-coated plates in a dose-dependent manner and that Cry5B binding to glycolipids was inhibited by the addition of LEC-8. LEC-8 is usually expressed strongly in the pharyngeal-intestinal valve and intestinal-rectal valve and is expressed weakly in intestine. However, when C. elegans were fed Escherichia coli expressing Cry5B, intestinal LEC-8::EGFP protein levels increased markedly. In contrast, LEC-8::EGFP expression triggered by Cry5B was reduced in toxin-resistant C. elegans mutants, which had mutations in genes involved in biosynthesis of glycolipids. Moreover, the LEC-8-deficient mutant was more susceptible to Cry5B than wild-type worms. These results suggest that the glycolipid-binding lectin LEC-8 contributes to host defense against bacterial infection by competitive binding to target glycolipid molecules. |
Volume | 284(39) |
Pages | 26493-501 |
Published | 2009-9-25 |
DOI | 10.1074/jbc.M109.038257 |
PII | S0021-9258(20)38480-5 |
PMID | 19635802 |
PMC | PMC2785337 |
MeSH | Animals Bacillus thuringiensis / metabolism Bacillus thuringiensis / physiology Bacillus thuringiensis Toxins Bacterial Proteins / metabolism Bacterial Proteins / toxicity Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / drug effects Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromatography, High Pressure Liquid Endotoxins / metabolism Endotoxins / toxicity Enzyme-Linked Immunosorbent Assay Galectins / genetics Galectins / metabolism* Glycolipids / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hemolysin Proteins / metabolism Hemolysin Proteins / toxicity Host-Pathogen Interactions Intestinal Mucosa / metabolism Mutation Protein Binding Protein Isoforms / genetics Protein Isoforms / metabolism Recombinant Proteins / metabolism |
IF | 4.238 |
Times Cited | 45 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
C.elegans | tm1477 |