| RRC ID |
64611
|
| 著者 |
Hirota E, Yan L, Tsunoda T, Ashida S, Fujime M, Shuin T, Miki T, Nakamura Y, Katagiri T.
|
| タイトル |
Genome-wide gene expression profiles of clear cell renal cell carcinoma: identification of molecular targets for treatment of renal cell carcinoma.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
In order to clarify the molecular mechanism involved in renal carcinogenesis, and to identify molecular targets for diagnosis and treatment, we analyzed genome-wide gene expression profiles of 15 surgical specimens of clear cell renal cell carcinoma (RCC), compared to normal renal cortex, using a combination of laser microbeam microdissection (LMM) with a cDNA microarray representing 27,648 genes. We identified 257 genes that were commonly up-regulated and 721 genes that were down-regulated in RCCs. None of top 24 up-regulated genes that showed most significant differences in informative RCC-cases were included in previous reports describing expression profiles of RCC using RNAs isolated from bulk tissues. These findings suggest that it is important to purify as much as possible the populations of cancerous and normal epithelial cells obtained from surgical specimens. Among the significantly-transactivated genes, we focused on Semaphorin 5B (SEMA5B) and knocked-down its expression in RCC cells by small-interfering RNA (siRNA). Effective down-regulation of its expression levels in RCC cells significantly attenuated RCC cell viability. In conclusion, our data should be helpful for a better understanding of the tumorigenesis of RCC and should contribute to the development of diagnostic tumor markers and molecular-targeting therapy for patients with RCC.
|
| 巻・号 |
29(4)
|
| ページ |
799-827
|
| 公開日 |
2006-10-1
|
| PMID |
16964377
|
| MeSH |
Adult
Aged
Biomarkers, Tumor / genetics*
Carcinoma, Renal Cell / diagnosis
Carcinoma, Renal Cell / genetics*
Carcinoma, Renal Cell / therapy
Cell Line, Tumor
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic*
Genes, Neoplasm / genetics
Humans
Kidney Neoplasms / diagnosis
Kidney Neoplasms / genetics*
Kidney Neoplasms / therapy
Male
Membrane Glycoproteins / antagonists & inhibitors
Membrane Glycoproteins / genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis
RNA Interference
RNA, Small Interfering / genetics
Semaphorins / antagonists & inhibitors
Semaphorins / genetics*
Transcriptional Activation
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
OS-RC-2(RCB0735)
TUHR10TKB(RCB1275)
TUHR14TKB(RCB1383) |
