論文 - 詳細
| RRC ID | 64627 |
|---|---|
| 著者 | Nakase I, Noguchi K, Fujii I, Futaki S. |
| タイトル | Vectorization of biomacromolecules into cells using extracellular vesicles with enhanced internalization induced by macropinocytosis. |
| ジャーナル | Sci Rep |
| Abstract |
Extracellular vesicles (EVs, exosomes) are approximately 30- to 200-nm-long vesicles that have received increased attention due to their role in cell-to-cell communication. Although EVs are highly anticipated to be a next-generation intracellular delivery tool because of their pharmaceutical advantages, including non-immunogenicity, their cellular uptake efficacy is low because of the repulsion of EVs and negatively charged cell membranes and size limitations in endocytosis. Here, we demonstrate a methodology for achieving enhanced cellular EV uptake using arginine-rich cell-penetrating peptides (CPPs) to induce active macropinocytosis. The induction of macropinocytosis via a simple modification to the exosomal membrane using stearylated octaarginine, which is a representative CPP, significantly enhanced the cellular EV uptake efficacy. Consequently, effective EV-based intracellular delivery of an artificially encapsulated ribosome-inactivating protein, saporin, in EVs was attained. |
| 巻・号 | 6 |
| ページ | 34937 |
| 公開日 | 2016-10-17 |
| DOI | 10.1038/srep34937 |
| PII | srep34937 |
| PMID | 27748399 |
| PMC | PMC5066177 |
| MeSH | Animals Arginine / chemistry CHO Cells Cell Count Cell Line, Tumor Cell Membrane / metabolism Cell-Penetrating Peptides / metabolism Cricetinae Cricetulus Endocytosis Exosomes / metabolism* Extracellular Vesicles / metabolism Fluorescent Dyes / chemistry HeLa Cells Humans Macromolecular Substances* Microscopy, Electron, Transmission Oligopeptides / chemistry Peptides / chemistry Pinocytosis* Ribosome Inactivating Proteins, Type 1 / chemistry* Ribosomes / chemistry* Saporins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |