RRC ID 64627
著者 Nakase I, Noguchi K, Fujii I, Futaki S.
タイトル Vectorization of biomacromolecules into cells using extracellular vesicles with enhanced internalization induced by macropinocytosis.
ジャーナル Sci Rep
Abstract Extracellular vesicles (EVs, exosomes) are approximately 30- to 200-nm-long vesicles that have received increased attention due to their role in cell-to-cell communication. Although EVs are highly anticipated to be a next-generation intracellular delivery tool because of their pharmaceutical advantages, including non-immunogenicity, their cellular uptake efficacy is low because of the repulsion of EVs and negatively charged cell membranes and size limitations in endocytosis. Here, we demonstrate a methodology for achieving enhanced cellular EV uptake using arginine-rich cell-penetrating peptides (CPPs) to induce active macropinocytosis. The induction of macropinocytosis via a simple modification to the exosomal membrane using stearylated octaarginine, which is a representative CPP, significantly enhanced the cellular EV uptake efficacy. Consequently, effective EV-based intracellular delivery of an artificially encapsulated ribosome-inactivating protein, saporin, in EVs was attained.
巻・号 6
ページ 34937
公開日 2016-10-17
DOI 10.1038/srep34937
PII srep34937
PMID 27748399
PMC PMC5066177
MeSH Animals Arginine / chemistry CHO Cells Cell Count Cell Line, Tumor Cell Membrane / metabolism Cell-Penetrating Peptides / metabolism Cricetinae Cricetulus Endocytosis Exosomes / metabolism* Extracellular Vesicles / metabolism Fluorescent Dyes / chemistry HeLa Cells Humans Macromolecular Substances* Microscopy, Electron, Transmission Oligopeptides / chemistry Peptides / chemistry Pinocytosis* Ribosome Inactivating Proteins, Type 1 / chemistry* Ribosomes / chemistry* Saporins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 3.998
リソース情報
ヒト・動物細胞 HeLa