論文 - 詳細
| RRC ID | 64762 |
|---|---|
| 著者 | Ma M, Kler S, Pan YA. |
| タイトル | Structural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mapping. |
| ジャーナル | Front Neural Circuits |
| Abstract |
The unique combination of small size, translucency, and powerful genetic tools makes larval zebrafish a uniquely useful vertebrate system to investigate normal and pathological brain structure and function. While functional connectivity can now be assessed by optical imaging (via fluorescent calcium or voltage reporters) at the whole-brain scale, it remains challenging to systematically determine structural connections and identify connectivity changes during development or disease. To address this, we developed Tracer with Restricted Anterograde Spread (TRAS), a novel vesicular stomatitis virus (VSV)-based neural circuit labeling approach. TRAS makes use of replication-incompetent VSV (VSVΔG) and a helper virus (lentivirus) to enable anterograde transneuronal spread between efferent axons and their direct postsynaptic targets but restricts further spread to downstream areas. We integrated TRAS with the Z-Brain zebrafish 3D atlas for quantitative connectivity analysis and identified targets of the retinal and habenular efferent projections, in patterns consistent with previous reports. We compared retinofugal connectivity patterns between wild-type and down syndrome cell adhesion molecule-like 1 (dscaml1) mutant zebrafish and revealed differences in topographical distribution. These results demonstrate the utility of TRAS for quantitative structural connectivity analysis that would be valuable for detecting novel efferent targets and mapping connectivity changes underlying neurological or behavioral deficits. |
| 巻・号 | 13 |
| ページ | 85 |
| 公開日 | 2019-1-1 |
| DOI | 10.3389/fncir.2019.00085 |
| PMID | 32038180 |
| PMC | PMC6989443 |
| MeSH | Animals Animals, Genetically Modified Brain / cytology Brain / diagnostic imaging* Brain Chemistry / physiology Brain Mapping / methods* HEK293 Cells Humans Nerve Net / chemistry* Nerve Net / cytology Nerve Net / diagnostic imaging* Neuroanatomical Tract-Tracing Techniques / methods* Neurons / chemistry* Neurons / physiology Zebrafish |
| IF | 3.156 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vglut2a:GFP) |