RRC ID 64770
Author Liu Z, Kimura Y, Higashijima SI, Hildebrand DGC, Morgan JL, Bagnall MW.
Title Central Vestibular Tuning Arises from Patterned Convergence of Otolith Afferents.
Journal Neuron
Abstract As sensory information moves through the brain, higher-order areas exhibit more complex tuning than lower areas. Though models predict that complexity arises via convergent inputs from neurons with diverse response properties, in most vertebrate systems, convergence has only been inferred rather than tested directly. Here, we measure sensory computations in zebrafish vestibular neurons across multiple axes in vivo. We establish that whole-cell physiological recordings reveal tuning of individual vestibular afferent inputs and their postsynaptic targets. Strong, sparse synaptic inputs can be distinguished by their amplitudes, permitting analysis of afferent convergence in vivo. An independent approach, serial-section electron microscopy, supports the inferred connectivity. We find that afferents with similar or differing preferred directions converge on central vestibular neurons, conferring more simple or complex tuning, respectively. Together, these results provide a direct, quantifiable demonstration of feedforward input convergence in vivo.
Volume 108(4)
Pages 748-762.e4
Published 2020-11-25
DOI 10.1016/j.neuron.2020.08.019
PII S0896-6273(20)30650-4
PMID 32937099
PMC PMC7704800
MeSH Animals Electric Stimulation Evoked Potentials, Somatosensory / physiology Gene Knock-In Techniques Microscopy, Electron Neurons / physiology Neurons / ultrastructure Neurons, Afferent / physiology* Neurons, Afferent / ultrastructure Otolithic Membrane / physiology* Vestibular Nuclei / physiology* Vestibular Nuclei / ultrastructure Zebrafish
IF 14.415
Zebrafish Tg(nefma:Gal4)