論文 - 詳細
| RRC ID | 65046 |
|---|---|
| 著者 | Nakase I, Noguchi K, Aoki A, Takatani-Nakase T, Fujii I, Futaki S. |
| タイトル | Arginine-rich cell-penetrating peptide-modified extracellular vesicles for active macropinocytosis induction and efficient intracellular delivery. |
| ジャーナル | Sci Rep |
| Abstract |
Extracellular vesicles (EVs) including exosomes have been shown to play crucial roles in cell-to-cell communication because of their ability to carry biofunctional molecules (e.g., microRNAs and enzymes). EVs also have pharmaceutical advantages and are highly anticipated to be a next-generation intracellular delivery tool. Here, we demonstrate an experimental technique that uses arginine-rich cell-penetrating peptide (CPP)-modified EVs to induce active macropinocytosis for effective cellular EV uptake. Modification of arginine-rich CPPs on the EV membrane resulted in the activation of the macropinocytosis pathway, and the number of arginine residues in the peptide sequences affected the cellular EV uptake efficiency. Consequently, the ribosome-inactivating protein saporin-encapsulated EVs modified with hexadeca-arginine (R16) peptide effectively attained anti-cancer activity. |
| 巻・号 | 7(1) |
| ページ | 1991 |
| 公開日 | 2017-5-16 |
| DOI | 10.1038/s41598-017-02014-6 |
| PII | 10.1038/s41598-017-02014-6 |
| PMID | 28512335 |
| PMC | PMC5434003 |
| MeSH | Animals Arginine / metabolism* CHO Cells Cell Line, Tumor Cell Membrane / metabolism Cell-Penetrating Peptides / chemistry Cell-Penetrating Peptides / metabolism* Cricetulus Endocytosis Exosomes / metabolism Extracellular Vesicles / metabolism* Humans Models, Molecular Pinocytosis* Protein Conformation Protein Transport Saporins / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |