RRC ID 65048
著者 Ge Z, Cheng Z, Yang X, Huo X, Wang N, Wang H, Wang C, Gu D, Zhao F, Yao M, Fan J, Qin W.
タイトル Long noncoding RNA SchLAH suppresses metastasis of hepatocellular carcinoma through interacting with fused in sarcoma.
ジャーナル Cancer Sci
Abstract Emerging evidence has indicated that deregulation of long non-coding RNAs (lncRNAs) can contribute to the progression and metastasis of human cancer, including hepatocellular carcinoma (HCC). However, the roles of most lncRNAs in HCC remain largely unknown. Here we found a long noncoding RNA termed SchLAH (seven chromosome locus associated with HCC; also called BC035072) was generally downregulated in HCC. Low expression of SchLAH was significantly correlated with shorter overall survival of HCC patients. In vitro and in vivo assays indicated that overexpression of SchLAH inhibited the migration and lung metastasis of HCC cells. Knockdown of SchLAH by siRNA pool promoted the migration of HCC cells. RNA pull-down and RNA immunoprecipitation assays demonstrated SchLAH physically interacted with fused in sarcoma (FUS). PCR array analysis showed that RhoA and Rac1 were the downstream effector molecules of SchLAH during HCC metastasis. Knockdown of FUS rescued the mRNA levels of RhoA and Rac1 that were repressed by SchLAH. These results suggest that SchLAH may suppress the metastasis of HCC cells by interacting with FUS, which indicates potential of SchLAH for the prognosis and treatment of HCC.
巻・号 108(4)
ページ 653-662
公開日 2017-4-1
DOI 10.1111/cas.13200
PMID 28196303
PMC PMC5406589
MeSH Animals Blotting, Western Carcinoma, Hepatocellular / genetics* Carcinoma, Hepatocellular / metabolism Carcinoma, Hepatocellular / pathology Cell Line, Tumor Cell Movement / genetics Gene Expression Regulation, Neoplastic* Hep G2 Cells Humans Liver Neoplasms / genetics* Liver Neoplasms / metabolism Liver Neoplasms / pathology Mice, Inbred BALB C Mice, Nude Neoplasm Metastasis Prognosis Protein Binding RNA Interference RNA, Long Noncoding / genetics* RNA, Long Noncoding / metabolism RNA-Binding Protein FUS / genetics* RNA-Binding Protein FUS / metabolism Reverse Transcriptase Polymerase Chain Reaction Transplantation, Heterologous rac1 GTP-Binding Protein / genetics rac1 GTP-Binding Protein / metabolism rhoA GTP-Binding Protein / genetics rhoA GTP-Binding Protein / metabolism
IF 4.966
リソース情報
ヒト・動物細胞 HuH-7