| RRC ID |
65130
|
| 著者 |
Koshizuka K, Nohata N, Hanazawa T, Kikkawa N, Arai T, Okato A, Fukumoto I, Katada K, Okamoto Y, Seki N.
|
| タイトル |
Deep sequencing-based microRNA expression signatures in head and neck squamous cell carcinoma: dual strands of pre-miR-150 as antitumor miRNAs.
|
| ジャーナル |
Oncotarget
|
| Abstract |
We adopted into RNA-sequencing technologies to construct the microRNA (miRNA) expression signature of head and neck squamous cell carcinoma (HNSCC). Our signature revealed that a total of 160 miRNAs (44 upregulated and 116 downregulated) were aberrantly expressed in cancer tissues. Expression of miR-150-5p (guide strand miRNA) and miR-150-3p (passenger strand miRNA) were significantly silenced in cancer tissues, suggesting both miRNAs act as antitumor miRNAs in HNSCC cells. Ectopic expression of mature miRNAs, miR-150-5p and miR-150-3p inhibited cancer cell aggressiveness. Low expression of miR-150-5p and miR-150-3p predicted significantly shorter overall survival in patients with HNSCC (P = 0.0091 and P = 0.0386) by Kaplan-Meier survival curves analyses. We identified that integrin α3 (ITGA3), integrin α6 (ITGA6), and tenascin C (TNC) were coordinately regulated by these miRNAs in HNSCC cells. Knockdown assays using siRNAs showed that ITGA3, ITGA6 and TNC acted as cancer promoting genes in HNSCC cells. Moreover, ITGA3, ITGA6, and TNC alterations were associated with significantly poorer overall survival (P = 0.0177, P = 0.0237, and P = 0.026, respectively). Dual strands of pre-150 (miR-150-5p and miR-150-3p) functioned as antitumor miRNAs based on the miRNA expression signature of HNSCC. Identification of antitumor miR-150-mediated RNA networks may provide novel insights into pathogenesis of HNSCC.
|
| 巻・号 |
8(18)
|
| ページ |
30288-30304
|
| 公開日 |
2017-5-2
|
| DOI |
10.18632/oncotarget.16327
|
| PII |
16327
|
| PMID |
28415821
|
| PMC |
PMC5444743
|
| MeSH |
Aged
Aged, 80 and over
Biomarkers, Tumor*
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation
Ectopic Gene Expression
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Head and Neck Neoplasms / genetics*
Head and Neck Neoplasms / metabolism
Head and Neck Neoplasms / pathology
High-Throughput Nucleotide Sequencing
Humans
Male
MicroRNAs / genetics*
Middle Aged
Neoplasm Grading
Neoplasm Staging
RNA Interference
RNA Precursors
RNA, Messenger / genetics
Squamous Cell Carcinoma of Head and Neck
Transcriptome*
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
HSC-3(RCB1975) |
