論文 - 詳細
| RRC ID | 65130 |
|---|---|
| 著者 | Koshizuka K, Nohata N, Hanazawa T, Kikkawa N, Arai T, Okato A, Fukumoto I, Katada K, Okamoto Y, Seki N. |
| タイトル | Deep sequencing-based microRNA expression signatures in head and neck squamous cell carcinoma: dual strands of pre-miR-150 as antitumor miRNAs. |
| ジャーナル | Oncotarget |
| Abstract |
We adopted into RNA-sequencing technologies to construct the microRNA (miRNA) expression signature of head and neck squamous cell carcinoma (HNSCC). Our signature revealed that a total of 160 miRNAs (44 upregulated and 116 downregulated) were aberrantly expressed in cancer tissues. Expression of miR-150-5p (guide strand miRNA) and miR-150-3p (passenger strand miRNA) were significantly silenced in cancer tissues, suggesting both miRNAs act as antitumor miRNAs in HNSCC cells. Ectopic expression of mature miRNAs, miR-150-5p and miR-150-3p inhibited cancer cell aggressiveness. Low expression of miR-150-5p and miR-150-3p predicted significantly shorter overall survival in patients with HNSCC (P = 0.0091 and P = 0.0386) by Kaplan-Meier survival curves analyses. We identified that integrin α3 (ITGA3), integrin α6 (ITGA6), and tenascin C (TNC) were coordinately regulated by these miRNAs in HNSCC cells. Knockdown assays using siRNAs showed that ITGA3, ITGA6 and TNC acted as cancer promoting genes in HNSCC cells. Moreover, ITGA3, ITGA6, and TNC alterations were associated with significantly poorer overall survival (P = 0.0177, P = 0.0237, and P = 0.026, respectively). Dual strands of pre-150 (miR-150-5p and miR-150-3p) functioned as antitumor miRNAs based on the miRNA expression signature of HNSCC. Identification of antitumor miR-150-mediated RNA networks may provide novel insights into pathogenesis of HNSCC. |
| 巻・号 | 8(18) |
| ページ | 30288-30304 |
| 公開日 | 2017-5-2 |
| DOI | 10.18632/oncotarget.16327 |
| PII | 16327 |
| PMID | 28415821 |
| PMC | PMC5444743 |
| MeSH | Aged Aged, 80 and over Biomarkers, Tumor* Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology Cell Line, Tumor Cell Movement / genetics Cell Proliferation Ectopic Gene Expression Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Head and Neck Neoplasms / genetics* Head and Neck Neoplasms / metabolism Head and Neck Neoplasms / pathology High-Throughput Nucleotide Sequencing Humans Male MicroRNAs / genetics* Middle Aged Neoplasm Grading Neoplasm Staging RNA Interference RNA Precursors RNA, Messenger / genetics Squamous Cell Carcinoma of Head and Neck Transcriptome* |
| IF | 5.168 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-3(RCB1975) |