RRC ID 65180
Author Pham TTH, Huang WY, Chen CS, Chiu WT, Chuang HS.
Title Effects of electrotactic exercise and antioxidant EUK-134 on oxidative stress relief in Caenorhabditis elegans.
Journal PLoS One
Abstract Antioxidant uptake and regular exercise are two well-acknowledged measures used for rejuvenation and oxidative stress elimination. Previous studies have revealed that moderate exercise mildly increases intracellular signaling oxidant levels and strengthens the ability of an organism to deal with escalating oxidative stress by upregulating antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase. Antioxidant supplementation directly scavenges intracellular reactive oxygen species (ROS) to reduce oxidative stress. However, research to understand the impacts of these enzymes on mitigating oxidative stress from the perspective of simple animals is limited. Herein, we show that exercise combined with antioxidant supplementation ameliorates the physiological phenotypes and markers of aging in wild-type and SOD/CAT-deficient Caenorhabditis elegans. We discovered that treated wild-type and gene-deficient worms show better survivorship, reproduction, and motility compared with their control counterparts. Assays of biochemical indices revealed that variations in sod-3 expression under different stress levels imply an inducible enzyme response resulting from exercise training and antioxidant supplementation. In addition, induced ROS resistance obtained from any type of treatment could persist for several days even after treatment cessation, thus suggesting a potential long-term antioxidative stress effect. Our findings confirm that exercise, antioxidant supplementation, and their combination could significantly improve the ability of C. elegans to withstand adverse stress. Our observations provide promising insights into future therapies of anti-oxidative stress in higher animals.
Volume 16(1)
Pages e0245474
Published 2021-1-1
DOI 10.1371/journal.pone.0245474
PII PONE-D-20-14315
PMID 33471830
PMC PMC7817057
MeSH Animals Antioxidants / pharmacology* Caenorhabditis elegans / drug effects* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Gene Expression Regulation, Enzymologic / drug effects Organometallic Compounds / pharmacology* Oxidative Stress / drug effects* Physical Conditioning, Animal Reactive Oxygen Species / metabolism Salicylates / pharmacology* Static Electricity* Superoxide Dismutase / metabolism
Resource
C.elegans tm760