| RRC ID |
65193
|
| 著者 |
Ma YC, Yang ZS, Ma LQ, Shu R, Zou CG, Zhang KQ.
|
| タイトル |
YAP in epithelium senses gut barrier loss to deploy defenses against pathogens.
|
| ジャーナル |
PLoS Pathog
|
| Abstract |
Pathogens commonly disrupt the intestinal epithelial barrier; however, how the epithelial immune system senses the loss of intestinal barrier as a danger signal to activate self-defense is unclear. Through an unbiased approach in the model nematode Caenorhabditis elegans, we found that the EGL-44/TEAD transcription factor and its transcriptional activator YAP-1/YAP (Yes-associated protein) were activated when the intestinal barrier was disrupted by infections with the pathogenic bacterium Pseudomonas aeruginosa PA14. Gene Ontology enrichment analysis of the genes containing the TEAD-binding sites revealed that "innate immune response" and "defense response to Gram-negative bacterium" were two top significantly overrepresented terms. Genetic inactivation of yap-1 and egl-44 significantly reduced the survival rate and promoted bacterial accumulation in worms after bacterial infections. Furthermore, we found that disturbance of the E-cadherin-based adherens junction triggered the nuclear translocation and activation of YAP-1/YAP in the gut of worms. Although YAP is a major downstream effector of the Hippo signaling, our study revealed that the activation of YAP-1/YAP was independent of the Hippo pathway during disruption of intestinal barrier. After screening 10 serine/threonine phosphatases, we identified that PP2A phosphatase was involved in the activation of YAP-1/YAP after intestinal barrier loss induced by bacterial infections. Additionally, our study demonstrated that the function of YAP was evolutionarily conserved in mice. Our study highlights how the intestinal epithelium recognizes the loss of the epithelial barrier as a danger signal to deploy defenses against pathogens, uncovering an immune surveillance program in the intestinal epithelium.
|
| 巻・号 |
16(8)
|
| ページ |
e1008766
|
| 公開日 |
2020-8-1
|
| DOI |
10.1371/journal.ppat.1008766
|
| PII |
PPATHOGENS-D-19-02215
|
| PMID |
32857822
|
| PMC |
PMC7454999
|
| MeSH |
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Membrane Permeability*
Epithelial Cells / immunology*
Epithelial Cells / metabolism
Epithelial Cells / microbiology
Epithelial Cells / pathology
Gastrointestinal Microbiome / immunology*
Mice
Salmonella Infections, Animal / immunology*
Salmonella Infections, Animal / metabolism
Salmonella Infections, Animal / microbiology
Salmonella Infections, Animal / pathology
Salmonella typhimurium / immunology*
Signal Transduction
YAP-Signaling Proteins
|
| リソース情報 |
| 線虫 |
tm1416 |
