Reference - Detail
| RRC ID | 65203 |
|---|---|
| Author | Suen KM, Braukmann F, Butler R, Bensaddek D, Akay A, Lin CC, Milonaitytė D, Doshi N, Sapetschnig A, Lamond A, Ladbury JE, Miska EA. |
| Title | DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans. |
| Journal | Nat Commun |
| Abstract |
Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle P granule in Caenorhabditis elegans. Proteomic analysis of the PIWI protein PRG-1 reveals an interaction with the constitutive P granule protein DEPS-1. DEPS-1 is not required for piRNA biogenesis but piRNA-dependent silencing: deps-1 mutants fail to produce the secondary endo-siRNAs required for the silencing of piRNA targets. We identify a motif on DEPS-1 which mediates a direct interaction with PRG-1. DEPS-1 and PRG-1 form intertwining clusters to build elongated condensates in vivo which are dependent on the Piwi-interacting motif of DEPS-1. Additionally, we identify EDG-1 as an interactor of DEPS-1 and PRG-1. Our study reveals how specific protein-protein interactions drive the spatial organisation and piRNA-dependent silencing within membraneless organelles. |
| Volume | 11(1) |
| Pages | 4242 |
| Published | 2020-8-25 |
| DOI | 10.1038/s41467-020-18089-1 |
| PII | 10.1038/s41467-020-18089-1 |
| PMID | 32843637 |
| PMC | PMC7447803 |
| MeSH | Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism* Binding Sites Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cytoplasmic Granules / metabolism Gene Silencing* Germ Cells / metabolism Mutation Protein Binding Proteomics RNA Interference RNA, Double-Stranded / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* |
| Resource | |
| C.elegans | tm1358 |