論文 - 詳細
| RRC ID | 65210 |
|---|---|
| 著者 | Zhang A, Guan Z, Ockerman K, Dong P, Guo J, Wang Z, Yan D. |
| タイトル | Regulation of glial size by eicosapentaenoic acid through a novel Golgi apparatus mechanism. |
| ジャーナル | PLoS Biol |
| Abstract |
Coordination of cell growth is essential for the development of the brain, but the molecular mechanisms underlying the regulation of glial and neuronal size are poorly understood. To investigate the mechanisms involved in glial size regulation, we used Caenorhabditis elegans amphid sheath (AMsh) glia as a model and show that a conserved cis-Golgi membrane protein eas-1/GOLT1B negatively regulates glial growth. We found that eas-1 inhibits a conserved E3 ubiquitin ligase rnf-145/RNF145, which, in turn, promotes nuclear activation of sbp-1/ SREBP, a key regulator of sterol and fatty acid synthesis, to restrict cell growth. At early developmental stages, rnf-145 in the cis-Golgi network inhibits sbp-1 activation to promote the growth of glia, and when animals reach the adult stage, this inhibition is released through an eas-1-dependent shuttling of rnf-145 from the cis-Golgi to the trans-Golgi network to stop glial growth. Furthermore, we identified long-chain polyunsaturated fatty acids (LC-PUFAs), especially eicosapentaenoic acid (EPA), as downstream products of the eas-1-rnf-145-sbp-1 pathway that functions to prevent the overgrowth of glia. Together, our findings reveal a novel and potentially conserved mechanism underlying glial size control. |
| 巻・号 | 18(12) |
| ページ | e3001051 |
| 公開日 | 2020-12-1 |
| DOI | 10.1371/journal.pbio.3001051 |
| PII | PBIOLOGY-D-20-02013 |
| PMID | 33370778 |
| PMC | PMC7793280 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Cell Size / drug effects Eicosapentaenoic Acid / metabolism Eicosapentaenoic Acid / pharmacology* Fatty Acids, Unsaturated / metabolism Golgi Apparatus / drug effects Golgi Apparatus / metabolism* Neuroglia / drug effects Neuroglia / metabolism* Ubiquitin-Protein Ligases / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6312 |