論文 - 詳細
| RRC ID | 65221 |
|---|---|
| 著者 | Farboud B, Novak CS, Nicoll M, Quiogue A, Meyer BJ. |
| タイトル | Dose-dependent action of the RNA binding protein FOX-1 to relay X-chromosome number and determine C. elegans sex. |
| ジャーナル | Elife |
| Abstract |
We demonstrate how RNA binding protein FOX-1 functions as a dose-dependent X-signal element to communicate X-chromosome number and thereby determine nematode sex. FOX-1, an RNA recognition motif protein, triggers hermaphrodite development in XX embryos by causing non-productive alternative pre-mRNA splicing of xol-1, the master sex-determination switch gene that triggers male development in XO embryos. RNA binding experiments together with genome editing demonstrate that FOX-1 binds to multiple GCAUG and GCACG motifs in a xol-1 intron, causing intron retention or partial exon deletion, thereby eliminating male-determining XOL-1 protein. Transforming all motifs to GCAUG or GCACG permits accurate alternative splicing, demonstrating efficacy of both motifs. Mutating subsets of both motifs partially alleviates non-productive splicing. Mutating all motifs blocks it, as does transforming them to low-affinity GCUUG motifs. Combining multiple high-affinity binding sites with the twofold change in FOX-1 concentration between XX and XO embryos achieves dose-sensitivity in splicing regulation to determine sex. |
| 巻・号 | 9 |
| 公開日 | 2020-12-29 |
| DOI | 10.7554/eLife.62963 |
| PII | 62963 |
| PMID | 33372658 |
| PMC | PMC7787662 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Female Introns / genetics Introns / physiology Male RNA-Binding Proteins / metabolism RNA-Binding Proteins / physiology* Sex Determination Processes X Chromosome / genetics* |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm611 |