RRC ID 65232
Author Kelley CA, De Henau S, Bell L, Dansen TB, Cram EJ.
Title Redox signaling modulates Rho activity and tissue contractility in the Caenorhabditis elegans spermatheca.
Journal Mol Biol Cell
Abstract Actomyosin-based contractility in smooth muscle and nonmuscle cells is regulated by signaling through the small GTPase Rho and by calcium-activated pathways. We use the myoepithelial cells of the Caenorhabditis elegans spermatheca to study the mechanisms of coordinated myosin activation in vivo. Here, we show that redox signaling modulates RHO-1/Rho activity in this contractile tissue. Exogenously added as well as endogenously generated hydrogen peroxide decreases spermathecal contractility by inhibition of RHO-1, which depends on a conserved cysteine in its nucleotide binding site (C20). Further, we identify an endogenous gradient of H2O2 across the spermathecal tissue, which depends on the activity of cytosolic superoxide dismutase, SOD-1. Collectively, we show that SOD-1-mediated H2O2 production regulates the redox environment and fine tunes Rho activity across the spermatheca through oxidation of RHO-1 C20.
Volume 31(14)
Pages 1486-1497
Published 2020-7-1
DOI 10.1091/mbc.E20-04-0236
PMID 32374641
PMC PMC7359568
MeSH Actin Cytoskeleton / metabolism Actomyosin / metabolism Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Calcium / metabolism Epithelial Cells / metabolism* Hydrogen Peroxide / metabolism Muscle Cells / metabolism Muscle Contraction / physiology* Muscle, Smooth / metabolism Muscle, Smooth / physiology Oxidation-Reduction Signal Transduction Superoxide Dismutase / metabolism rho GTP-Binding Proteins / metabolism* rho-Associated Kinases / metabolism
Resource
C.elegans tm776 tm1146