論文 - 詳細
| RRC ID | 65232 |
|---|---|
| 著者 | Kelley CA, De Henau S, Bell L, Dansen TB, Cram EJ. |
| タイトル | Redox signaling modulates Rho activity and tissue contractility in the Caenorhabditis elegans spermatheca. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Actomyosin-based contractility in smooth muscle and nonmuscle cells is regulated by signaling through the small GTPase Rho and by calcium-activated pathways. We use the myoepithelial cells of the Caenorhabditis elegans spermatheca to study the mechanisms of coordinated myosin activation in vivo. Here, we show that redox signaling modulates RHO-1/Rho activity in this contractile tissue. Exogenously added as well as endogenously generated hydrogen peroxide decreases spermathecal contractility by inhibition of RHO-1, which depends on a conserved cysteine in its nucleotide binding site (C20). Further, we identify an endogenous gradient of H2O2 across the spermathecal tissue, which depends on the activity of cytosolic superoxide dismutase, SOD-1. Collectively, we show that SOD-1-mediated H2O2 production regulates the redox environment and fine tunes Rho activity across the spermatheca through oxidation of RHO-1 C20. |
| 巻・号 | 31(14) |
| ページ | 1486-1497 |
| 公開日 | 2020-7-1 |
| DOI | 10.1091/mbc.E20-04-0236 |
| PMID | 32374641 |
| PMC | PMC7359568 |
| MeSH | Actin Cytoskeleton / metabolism Actomyosin / metabolism Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Calcium / metabolism Epithelial Cells / metabolism* Hydrogen Peroxide / metabolism Muscle Cells / metabolism Muscle Contraction / physiology* Muscle, Smooth / metabolism Muscle, Smooth / physiology Oxidation-Reduction Signal Transduction Superoxide Dismutase / metabolism rho GTP-Binding Proteins / metabolism* rho-Associated Kinases / metabolism |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm776 tm1146 |