RRC ID |
65279
|
Author |
Charest J, Daniele T, Wang J, Bykov A, Mandlbauer A, Asparuhova M, Röhsner J, Gutiérrez-Pérez P, Cochella L.
|
Title |
Combinatorial Action of Temporally Segregated Transcription Factors.
|
Journal |
Dev Cell
|
Abstract |
Combinatorial action of transcription factors (TFs) with partially overlapping expression is a widespread strategy to generate novel gene-expression patterns and, thus, cellular diversity. Known mechanisms underlying combinatorial activity require co-expression of TFs within the same cell. Here, we describe the mechanism by which two TFs that are never co-expressed generate a new, intersectional expression pattern in C. elegans embryos: lineage-specific priming of a gene by a transiently expressed TF generates a unique intersection with a second TF acting on the same gene four cell divisions later; the second TF is expressed in multiple cells but only activates transcription in those where priming occurred. Early induction of active transcription is necessary and sufficient to establish a competent state, maintained by broadly expressed regulators in the absence of the initial trigger. We uncover additional cells diversified through this mechanism. Our findings define a mechanism for combinatorial TF activity with important implications for generation of cell-type diversity.
|
Volume |
55(4)
|
Pages |
483-499.e7
|
Published |
2020-11-23
|
DOI |
10.1016/j.devcel.2020.09.002
|
PII |
S1534-5807(20)30672-9
|
PMID |
33002421
|
PMC |
PMC7704111
|
MeSH |
Animals
Caenorhabditis elegans / cytology
Caenorhabditis elegans / embryology
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism
Cell Lineage
Embryo, Nonmammalian / metabolism
Gene Expression Regulation
Genetic Loci
Neurons / metabolism
Protein Binding
Time Factors
Transcription Factors / metabolism*
Transcription, Genetic
|
Resource |
C.elegans |
tm314
tm581 |