RRC ID 65279
Author Charest J, Daniele T, Wang J, Bykov A, Mandlbauer A, Asparuhova M, Röhsner J, Gutiérrez-Pérez P, Cochella L.
Title Combinatorial Action of Temporally Segregated Transcription Factors.
Journal Dev Cell
Abstract Combinatorial action of transcription factors (TFs) with partially overlapping expression is a widespread strategy to generate novel gene-expression patterns and, thus, cellular diversity. Known mechanisms underlying combinatorial activity require co-expression of TFs within the same cell. Here, we describe the mechanism by which two TFs that are never co-expressed generate a new, intersectional expression pattern in C. elegans embryos: lineage-specific priming of a gene by a transiently expressed TF generates a unique intersection with a second TF acting on the same gene four cell divisions later; the second TF is expressed in multiple cells but only activates transcription in those where priming occurred. Early induction of active transcription is necessary and sufficient to establish a competent state, maintained by broadly expressed regulators in the absence of the initial trigger. We uncover additional cells diversified through this mechanism. Our findings define a mechanism for combinatorial TF activity with important implications for generation of cell-type diversity.
Volume 55(4)
Pages 483-499.e7
Published 2020-11-23
DOI 10.1016/j.devcel.2020.09.002
PII S1534-5807(20)30672-9
PMID 33002421
PMC PMC7704111
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Cell Lineage Embryo, Nonmammalian / metabolism Gene Expression Regulation Genetic Loci Neurons / metabolism Protein Binding Time Factors Transcription Factors / metabolism* Transcription, Genetic
Resource
C.elegans tm314 tm581