Reference - Detail
| RRC ID | 65303 |
|---|---|
| Author | Ohnishi K, Saito S, Miura T, Ohta A, Tominaga M, Sokabe T, Kuhara A. |
| Title | OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation. |
| Journal | Sci Rep |
| Abstract |
Caenorhabditis elegans (C. elegans) exhibits cold tolerance and temperature acclimatisation regulated by a small number of head sensory neurons, such as the ADL temperature-sensing neurons that express three transient receptor potential vanilloid (TRPV) channel subunits, OSM-9, OCR-2, and OCR-1. Here, we show that an OSM-9/OCR-2 regulates temperature acclimatisation and acts as an accessorial warmth-sensing receptor in ADL neurons. Caenorhabditis elegans TRPV channel mutants showed abnormal temperature acclimatisation. Ectopic expression of OSM-9 and OCR-2 in non-warming-responsive gustatory neurons in C. elegans and Xenopus oocytes revealed that OSM-9 and OCR-2 cooperatively responded to warming; however, neither TRPV subunit alone was responsive to warming. A warming-induced OSM-9/OCR-2-mediated current was detectable in Xenopus oocytes, yet ADL in osm-9 ocr-2 double mutant responds to warming; therefore, an OSM-9/OCR-2 TRPV channel and as yet unidentified temperature receptor might coordinate transmission of temperature signalling in ADL temperature-sensing neurons. This study demonstrates direct sensation of warming by TRPV channels in C. elegans. |
| Volume | 10(1) |
| Pages | 18566 |
| Published | 2020-10-29 |
| DOI | 10.1038/s41598-020-75302-3 |
| PII | 10.1038/s41598-020-75302-3 |
| PMID | 33122746 |
| PMC | PMC7596061 |
| MeSH | Acclimatization / genetics* Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Mutation / genetics Nerve Tissue Proteins / genetics* Oocytes / physiology Sensation / genetics Sensory Receptor Cells / physiology Signal Transduction / genetics TRPV Cation Channels / genetics* Temperature Xenopus / genetics |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
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| C.elegans | tm6403 |