論文 - 詳細
| RRC ID | 65380 |
|---|---|
| 著者 | Hsu TY, Zhang B, L'Etoile ND, Juang BT. |
| タイトル | C. elegans orthologs MUT-7/CeWRN-1 of Werner syndrome protein regulate neuronal plasticity. |
| ジャーナル | Elife |
| Abstract |
Caenorhabditis elegans expresses human Werner syndrome protein (WRN) orthologs as two distinct proteins: MUT-7, with a 3'-5' exonuclease domain, and CeWRN-1, with helicase domains. How these domains cooperate remains unclear. Here, we demonstrate the different contributions of MUT-7 and CeWRN-1 to 22G small interfering RNA (siRNA) synthesis and the plasticity of neuronal signaling. MUT-7 acts specifically in the cytoplasm to promote siRNA biogenesis and in the nucleus to associate with CeWRN-1. The import of siRNA by the nuclear Argonaute NRDE-3 promotes the loading of the heterochromatin-binding protein HP1 homolog HPL-2 onto specific loci. This heterochromatin complex represses the gene expression of the guanylyl cyclase ODR-1 to direct olfactory plasticity in C. elegans. Our findings suggest that the exonuclease and helicase domains of human WRN may act in concert to promote RNA-dependent loading into a heterochromatin complex, and the failure of this entire process reduces plasticity in postmitotic neurons. |
| 巻・号 | 10 |
| 公開日 | 2021-3-1 |
| DOI | 10.7554/eLife.62449 |
| PII | 62449 |
| PMID | 33646120 |
| PMC | PMC7946423 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / metabolism DNA Helicases / genetics DNA Helicases / metabolism* Exonucleases Heterochromatin Neuronal Plasticity RNA, Small Interfering / metabolism Werner Syndrome / genetics Werner Syndrome / metabolism* Werner Syndrome Helicase / genetics Werner Syndrome Helicase / metabolism* |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1489 |